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Literature DB >> 2559542

A region of basic amino-acid cluster in HIV-1 Tat protein is essential for trans-acting activity and nucleolar localization.

S Endo¹, S Kubota, H Siomi, A Adachi, S Oroszlan, M Maki, M Hatanaka.

Abstract

The trans-acting factor of human immunodeficiency virus (HIV), Tat, has a basic amino-acid cluster that is highly conserved among different HIV isolates. We have examined the effects of mutations in the basic region of Tat on its trans-acting activity and cellular localization. Introduction of a stop codon immediately preceding the basic region abolished the activity, while the truncated mutant with the basic region retained some activity. The basic region of Tat was replaceable with that of Rev (another trans-acting factor of HIV) but not with that of adenovirus Ela nor cellular enzyme. The result of immunofluorescence analysis revealed a correlation between the nuclear, especially nucleolar, accumulation and the activities of mutant Tat proteins.

Entities: Chemical Gene Species

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Year: 1989 PMID： 2559542 DOI： 10.1007/BF00125123

Source DB: PubMed Journal: Virus Genes ISSN： 0920-8569 Impact factor: 2.332

37 in total

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Authors: M Maki; E Takano; H Mori; A Sato; T Murachi; M Hatanaka
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Authors: S Wain-Hobson; P Sonigo; O Danos; S Cole; M Alizon
Journal: Cell Date: 1985-01 Impact factor: 41.582

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Authors: J Sodroski; R Patarca; C Rosen; F Wong-Staal; W Haseltine
Journal: Science Date: 1985-07-05 Impact factor: 47.728

4. The trans-activator gene of HTLV-III is essential for virus replication.

Authors: A G Fisher; M B Feinberg; S F Josephs; M E Harper; L M Marselle; G Reyes; M A Gonda; A Aldovini; C Debouk; R C Gallo
Journal: Nature Date: 1986 Mar 27-Apr 2 Impact factor: 49.962

5. Post-transcriptional regulation accounts for the trans-activation of the human T-lymphotropic virus type III.

Authors: C A Rosen; J G Sodroski; W C Goh; A I Dayton; J Lippke; W A Haseltine
Journal: Nature Date: 1986 Feb 13-19 Impact factor: 49.962

6. A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells.

Authors: M J Sleigh
Journal: Anal Biochem Date: 1986-07 Impact factor: 3.365

7. Cloning DNA restriction endonuclease fragments with protruding single-stranded ends.

Authors: R M Wartell; W S Reznikoff
Journal: Gene Date: 1980-05 Impact factor: 3.688

8. Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence.

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Journal: FEBS Lett Date: 1986-09-15 Impact factor: 4.124

9. Activation of double-stranded RNA-dependent kinase (dsl) by the TAR region of HIV-1 mRNA: a novel translational control mechanism.

Authors: I Edery; R Petryshyn; N Sonenberg
Journal: Cell Date: 1989-01-27 Impact factor: 41.582

10. Trans-activation of human immunodeficiency virus occurs via a bimodal mechanism.

Authors: B R Cullen
Journal: Cell Date: 1986-09-26 Impact factor: 41.582

24 in total

1. Inhibition of human immunodeficiency virus type 1 Rev function by a Rev mutant which interferes with nuclear/nucleolar localization of Rev.

Authors: S Kubota; R Furuta; M Maki; M Hatanaka
Journal: J Virol Date: 1992-04 Impact factor: 5.103

2. Functional comparison of the basic domains of the Tat proteins of human immunodeficiency virus types 1 and 2 in trans activation.

Authors: B Elangovan; T Subramanian; G Chinnadurai
Journal: J Virol Date: 1992-04 Impact factor: 5.103

3. Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization.

Authors: H Siomi; H Shida; M Maki; M Hatanaka
Journal: J Virol Date: 1990-04 Impact factor: 5.103

4. Mutational analysis of the equine infectious anemia virus Tat-responsive element.

Authors: M Carvalho; D Derse
Journal: J Virol Date: 1991-07 Impact factor: 5.103

5. Identification of lentivirus tat functional domains through generation of equine infectious anemia virus/human immunodeficiency virus type 1 tat gene chimeras.

Authors: R Carroll; L Martarano; D Derse
Journal: J Virol Date: 1991-07 Impact factor: 5.103

6. The dynamics of the alternatively spliced NOL7 gene products and role in nucleolar architecture.

Authors: Noa Kinor; Yaron Shav-Tal
Journal: Nucleus Date: 2011 May-Jun Impact factor: 4.197

7. Effects of human chromosome 12 on interactions between Tat and TAR of human immunodeficiency virus type 1.

Authors: A Alonso; T P Cujec; B M Peterlin
Journal: J Virol Date: 1994-10 Impact factor: 5.103

8. The number of positively charged amino acids in the basic domain of Tat is critical for trans-activation and complex formation with TAR RNA.

Authors: U Delling; S Roy; M Sumner-Smith; R Barnett; L Reid; C A Rosen; N Sonenberg
Journal: Proc Natl Acad Sci U S A Date: 1991-07-15 Impact factor: 11.205

9. Heparin-mimicking sulfonic acid polymers as multitarget inhibitors of human immunodeficiency virus type 1 Tat and gp120 proteins.

Authors: Antonella Bugatti; Chiara Urbinati; Cosetta Ravelli; Erik De Clercq; Sandra Liekens; Marco Rusnati
Journal: Antimicrob Agents Chemother Date: 2007-04-23 Impact factor: 5.191

10. Use of a human immunodeficiency virus type 1 Rev mutant without nucleolar dysfunction as a candidate for potential AIDS therapy.

Authors: R A Furuta; S Kubota; M Maki; Y Miyazaki; T Hattori; M Hatanaka
Journal: J Virol Date: 1995-03 Impact factor: 5.103