Literature DB >> 25585802

Cell-free synthesis of SecYEG translocon as the fundamental protein transport machinery.

Hideaki Matsubayashi1, Yutetsu Kuruma, Takuya Ueda.   

Abstract

The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.

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Year:  2014        PMID： 25585802     DOI： 10.1007/s11084-014-9389-y

Source DB:  PubMed          Journal:  Orig Life Evol Biosph        ISSN： 0169-6149            Impact factor:   1.950


  17 in total

1.  Cell-free translation reconstituted with purified components.

Authors:  Y Shimizu; A Inoue; Y Tomari; T Suzuki; T Yokogawa; K Nishikawa; T Ueda
Journal:  Nat Biotechnol       Date:  2001-08       Impact factor: 54.908

2.  Functional analysis of membranous Fo-a subunit of F1Fo-ATP synthase by in vitro protein synthesis.

Authors:  Yutetsu Kuruma; Toshiharu Suzuki; Sakurako Ono; Masasuke Yoshida; Takuya Ueda
Journal:  Biochem J       Date:  2012-03-15       Impact factor: 3.857

3.  Gene expression within cell-sized lipid vesicles.

Authors:  Shin-ichiro M Nomura; Kanta Tsumoto; Tsutomu Hamada; Kazunari Akiyoshi; Yoichi Nakatani; Kenichi Yoshikawa
Journal:  Chembiochem       Date:  2003-11-07       Impact factor: 3.164

Review 4.  The Sec translocase.

Authors:  David J F du Plessis; Nico Nouwen; Arnold J M Driessen
Journal:  Biochim Biophys Acta       Date:  2010-08-27

5.  A vesicle bioreactor as a step toward an artificial cell assembly.

Authors:  Vincent Noireaux; Albert Libchaber
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-10       Impact factor: 11.205

6.  Cell-free expression and assembly of ATP synthase.

Authors:  Doreen Matthies; Stefan Haberstock; Friederike Joos; Volker Dötsch; Janet Vonck; Frank Bernhard; Thomas Meier
Journal:  J Mol Biol       Date:  2011-09-10       Impact factor: 5.469

7.  Diacylglycerol specifically blocks spontaneous integration of membrane proteins and allows detection of a factor-assisted integration.

Authors:  Yosuke Kawashima; Emi Miyazaki; Matthias Müller; Hajime Tokuda; Ken-ichi Nishiyama
Journal:  J Biol Chem       Date:  2008-07-09       Impact factor: 5.157

8.  A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells.

Authors:  Yutetsu Kuruma; Pasquale Stano; Takuya Ueda; Pier Luigi Luisi
Journal:  Biochim Biophys Acta       Date:  2008-11-05

9.  Did the last common ancestor have a biological membrane?

Authors:  Gáspár Jékely
Journal:  Biol Direct       Date:  2006-11-27       Impact factor: 4.540

10.  Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system.

Authors:  Kei Fujiwara; Tsutomu Katayama; Shin-Ichiro M Nomura
Journal:  Nucleic Acids Res       Date:  2013-06-03       Impact factor: 16.971
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  3 in total

1.  Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression.

Authors:  Paul J Focke; Christopher Hein; Beate Hoffmann; Kimberly Matulef; Frank Bernhard; Volker Dötsch; Francis I Valiyaveetil
Journal:  Biochemistry       Date:  2016-07-21       Impact factor: 3.162

Review 2.  Escherichia coli Extract-Based Cell-Free Expression System as an Alternative for Difficult-to-Obtain Protein Biosynthesis.

Authors:  Sviatlana Smolskaya; Yulia A Logashina; Yaroslav A Andreev
Journal:  Int J Mol Sci       Date:  2020-01-31       Impact factor: 5.923

3.  Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein.

Authors:  Laura R Blackholly; Nicola J Harris; Heather E Findlay; Paula J Booth
Journal:  Front Mol Biosci       Date:  2022-02-02
  3 in total

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