Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Cell-free translation reconstituted with purified components.

Literature DB >> 11479568

Cell-free translation reconstituted with purified components.

Y Shimizu¹, A Inoue, Y Tomari, T Suzuki, T Yokogawa, K Nishikawa, T Ueda.

Abstract

We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 microg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).

Mesh：

Substances：

Year: 2001 PMID： 11479568 DOI： 10.1038/90802

Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908

Keyword Cloud
Cited

466 in total

Cell-free translation reconstituted with purified components.

1. Flexizymes for genetic code reprogramming.

2. Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters.

3. Quick two-step RNA ligation employing periodate oxidation.

4. Programming peptidomimetic syntheses by translating genetic codes designed de novo.

5. In vitro selection for sense codon suppression.

Review 6. Evolutionary conservation of reactions in translation.

7. Principles of cell-free genetic circuit assembly.

8. Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification.

9. Profligate biotin synthesis in α-proteobacteria - a developing or degenerating regulatory system?

10. TIR Domain Proteins Are an Ancient Family of NAD⁺-Consuming Enzymes.