Yating Liu1, Yanping Wang2, Hongjuan Liu3, Jian'an Zhang4. 1. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China; Department of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. 2. Department of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China. 3. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China. Electronic address: liuhongjuan@tsinghua.edu.cn. 4. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China. Electronic address: zhangja@tsinghua.edu.cn.
Abstract
In recent years, energy crisis and environmental issues such as greenhouse effect, global warming, etc. has roused peoples' concern. Biodiesel, as renewable energy, has attracted much attention to deal with such problems. This work studied the lipid production by Rhodotorula glutinis with undetoxified corncob hydrolysate. The results indicated that R. glutinis had high tolerance to the inhibitors in corncob hydrolysate and it could utilize undetoxified corncob hydrolysate directly for lipid production. The cell grew well with undetoxified hydrolysate in the batch culture of 5L fermentor with the optimized C/N ratio of 75, lipid titer and lipid content reached 5.5g/L and 36.4%, respectively. High cell density culture with two-stage nitrogen feeding strategy was studied to enhance the lipid production, biomass, lipid concentration and lipid content of 70.8, 33.5g/L and 47.2% were obtained. The results indicated the potential application for lipid production by R. glutinis with corncob hydrolysate directly.
In recent years, energy crisis and environmental issues such as greenhouse effect, global warming, etc. has roused peoples' concern. Biodiesel, as renewable energy, has attracted much attention to deal with such problems. This work studied the n class="Chemical">lipid production by Rhodotorula glutinis with undetoxified corncob hydrolysate. The results indicated that R. glutinis had high tolerance to the inhibitors in corncob hydrolysate and it could utilize undetoxified corncob hydrolysate directly for lipid production. The cell grew well with undetoxified hydrolysate in the batch culture of 5L fermentor with the optimized C/N ratio of 75, lipid titer and lipid content reached 5.5g/L and 36.4%, respectively. High cell density culture with two-stage nitrogen feeding strategy was studied to enhance the lipid production, biomass, lipid concentration and lipid content of 70.8, 33.5g/L and 47.2% were obtained. The results indicated the potential application for lipid production by R. glutinis with corncob hydrolysate directly.
Authors: Cassamo U Mussagy; Helena F Ribeiro; Valeria C Santos-Ebinuma; Boelo Schuur; Jorge F B Pereira Journal: Appl Microbiol Biotechnol Date: 2022-10-18 Impact factor: 5.560