Michelle da Cunha Abreu Xavier1, Telma Teixeira Franco2. 1. Department of Bioprocess Engineering and Biotechnology, Federal University of Tocantins (UFT), Badejos Street 69-72, Jardim Cervilha, Gurupi, TO, 77404-970, Brazil. michellecax@mail.uft.edu.br. 2. Department of Process Engineering (DEPro), School of Chemical Engineering, State University of Campinas (UNICAMP), Albert Einstein Avenue, 500, Zeferino Vaz University City, Campinas, SP, 13083-852, Brazil.
Abstract
OBJECTIVE: The extraction of the hemicellulose fraction of sugarcane bagasse (SCB) by acid hydrolysis was evaluated in an autoclave and a Parr reactor aiming the application of the hydrolysate as a carbon source for lipid production by Lipomyces starkeyi. RESULTS: The hydrolysis that resulted in the highest sugar concentration was obtained by treatment in the Parr reactor (HHR) at 1.5% (m/v) H2SO4 and 120 °C for 20 min, reaching a hemicellulose conversion of approximately 82%. The adaptation of the yeast to the hydrolysate provided good fermentability and no lag phase. The fermentation of hemicellulose-derived sugars (HHR) by L. starkeyi resulted in a 27.8% (w/w) lipid content and YP/S of 0.16 g/l.h. Increasing the inoculum size increased the lipid content by approximately 61%, reaching 44.8% (w/w). CONCLUSION: The hemicellulose hydrolysate from SCB is a potential substrate for L. starkeyi to produce lipids for biodiesel synthesis based on the biorefinery concept.
OBJECTIVE: The extraction of the hemicellulose fraction of sugarcane bagasse (SCB) by acid hydrolysis was evaluated in an autoclave and a Parr reactor aiming the application of the hydrolysate as a carbon source for lipid production by Lipomyces starkeyi. RESULTS: The hydrolysis that resulted in the highest sugar concentration was obtained by treatment in the Parr reactor (HHR) at 1.5% (m/v) H2SO4 and 120 °C for 20 min, reaching a hemicellulose conversion of approximately 82%. The adaptation of the yeast to the hydrolysate provided good fermentability and no lag phase. The fermentation of hemicellulose-derived sugars (HHR) by L. starkeyi resulted in a 27.8% (w/w) lipid content and YP/S of 0.16 g/l.h. Increasing the inoculum size increased the lipid content by approximately 61%, reaching 44.8% (w/w). CONCLUSION: The hemicellulose hydrolysate from SCB is a potential substrate for L. starkeyi to produce lipids for biodiesel synthesis based on the biorefinery concept.
Authors: Larissa Canilha; Victor T O Santos; George J M Rocha; João B Almeida e Silva; Marco Giulietti; Silvio S Silva; Maria G A Felipe; André Ferraz; Adriane M F Milagres; Walter Carvalho Journal: J Ind Microbiol Biotechnol Date: 2011-01-06 Impact factor: 3.346
Authors: Larissa Canilha; Walter Carvalho; Maria das Graças de Almeida Felipe; João Batista de Almeida e Silva; Marco Giulietti Journal: Appl Biochem Biotechnol Date: 2009-10-04 Impact factor: 2.926