Pritam Mohanty1, Sridevi Padmanabhan2, Arun B Chitharanjan3. 1. Senior Lecturer, Department of Orthodontics & Dentofacial Orthopaedics, Kalinga Institute of Dental Sciences, KIIT University Bhubaneswar, Odisha, India . 2. Professor, Department of Orthodontics & Dentofacial Orthopaedics, Faculty of Dental Sciences, Sri Ramachandra University , Porur, Chennai, Tamil Nadu, India . 3. Professor & Head, Department of Orthodontics & Dentofacial Orthopaedics, Faculty of Dental Sciences, Sri Ramachandra University , Porur, Chennai, Tamil Nadu, India .
Abstract
OBJECTIVE: To evaluate and compare the Ca/P ratio of enamel samples around the orthodontic brackets for time periods of 0, 2 and 10 days in two groups (control group and study group). MATERIALS AND METHODS:Forty extracted teeth were randomly divided into control group and study group. All samples were demineralized and incubated in artificial saliva at 37°C for a period of 10 days after demineralization. During this phase the enamel samples in the study group were treated with remineralizing paste (NuproNusolution containing Novamin®-Dentsply) for 10 days. At the end of the incubation period, Ca/P ratios were analyzed for both the groupsby EDX analysis. Data obtained was subjected to statistical analysis using student t-test for paired samples and Student t- test for individual samples (p ≤ 0.05). RESULTS: It was found that the mean Ca/P ratio was significantly lower for the control group as compared to the study group (p-value < 0.05) after 10 d of incubation. CONCLUSION:Novamin(®) containing remineralization toothpaste showed significant remineralizing potential in inhibition of artificial enamel sub-surface lesion around bracket after 10 days of remineralization phase. EDX element analysis was found to be an efficient method to quantify the changes in mineral content of a sample during in vitro caries studies.
RCT Entities:
OBJECTIVE: To evaluate and compare the Ca/P ratio of enamel samples around the orthodontic brackets for time periods of 0, 2 and 10 days in two groups (control group and study group). MATERIALS AND METHODS: Forty extracted teeth were randomly divided into control group and study group. All samples were demineralized and incubated in artificial saliva at 37°C for a period of 10 days after demineralization. During this phase the enamel samples in the study group were treated with remineralizing paste (NuproNusolution containing Novamin®-Dentsply) for 10 days. At the end of the incubation period, Ca/P ratios were analyzed for both the groupsby EDX analysis. Data obtained was subjected to statistical analysis using student t-test for paired samples and Student t- test for individual samples (p ≤ 0.05). RESULTS: It was found that the mean Ca/P ratio was significantly lower for the control group as compared to the study group (p-value < 0.05) after 10 d of incubation. CONCLUSION: Novamin(®) containing remineralization toothpaste showed significant remineralizing potential in inhibition of artificial enamel sub-surface lesion around bracket after 10 days of remineralization phase. EDX element analysis was found to be an efficient method to quantify the changes in mineral content of a sample during in vitro caries studies.
Entities:
Keywords:
Demineralization; Orthodontics; White spot lesions