Pamela L Lutsey1, John H Eckfeldt2, Ejovwoke R Ogagarue3, Aaron R Folsom4, Erin D Michos5, Myron Gross2. 1. Division of Epidemiology and Community Health, University of Minnesota, 1300 So 2nd Street, Suite 300, Minneapolis, MN, United States. Electronic address: Lutsey@umn.edu. 2. Department of Laboratory Medicine and Pathology, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN, United States. 3. Department of Urology, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN, United States. 4. Division of Epidemiology and Community Health, University of Minnesota, 1300 So 2nd Street, Suite 300, Minneapolis, MN, United States. 5. Division of Cardiology, Johns Hopkins School of Medicine, 600 N. Wolfe Street, Baltimore, MD, United States.
Abstract
BACKGROUND: Little is known about the vitamin D3 epimer [3-epi-25(OH)D3], particularly in adults. We describe characteristics of the D3 epimer within the community-based ARIC cohort. METHODS: The vitamin D3 epimer, 25(OH)D3, and 25(OH)D2 were measured using LC-MS/MS in stored serum collected in 1990-1992 from 9,887 white and 3,221 black ARIC study participants, aged 46-70years. Cross-sectional characteristics were explored. RESULTS: Concentrations of the epimer were quantifiable (≥1.41ng/ml) in 33.4% of whites and 15.0% of blacks and made up on average 3.23% and 2.25% of total D3 [epimer+25(OH)D3] concentrations, respectively. Epimer levels were positively correlated with 25(OH)D3 in both whites (r=0.54) and blacks (r=0.36) and were unrelated to 25(OH)D2 concentrations. Overall, epimer levels were associated with participant characteristics in a manner similar to that typically observed for 25(OH)D3. Including the epimer in the calculation of total 25(OH)D resulted in approximately 2% of participants being reclassified from being clinically 25(OH)D deficient to having suboptimal levels. CONCLUSIONS: Low concentrations of the D3 epimer were present in adult serum and overall the epimer concentration is moderately correlated with the 25(OH)D3 concentration. The reclassification of participant's clinical 25(OH)D status upon inclusion of the epimer was minimal.
BACKGROUND: Little is known about the vitamin D3 epimer [3-epi-25(OH)D3], particularly in adults. We describe characteristics of the D3 epimer within the community-based ARIC cohort. METHODS: The vitamin D3 epimer, 25(OH)D3, and 25(OH)D2 were measured using LC-MS/MS in stored serum collected in 1990-1992 from 9,887 white and 3,221 black ARIC study participants, aged 46-70years. Cross-sectional characteristics were explored. RESULTS: Concentrations of the epimer were quantifiable (≥1.41ng/ml) in 33.4% of whites and 15.0% of blacks and made up on average 3.23% and 2.25% of total D3 [epimer+25(OH)D3] concentrations, respectively. Epimer levels were positively correlated with 25(OH)D3 in both whites (r=0.54) and blacks (r=0.36) and were unrelated to 25(OH)D2 concentrations. Overall, epimer levels were associated with participant characteristics in a manner similar to that typically observed for 25(OH)D3. Including the epimer in the calculation of total 25(OH)D resulted in approximately 2% of participants being reclassified from being clinically 25(OH)D deficient to having suboptimal levels. CONCLUSIONS: Low concentrations of the D3 epimer were present in adult serum and overall the epimer concentration is moderately correlated with the 25(OH)D3 concentration. The reclassification of participant's clinical25(OH)D status upon inclusion of the epimer was minimal.
Authors: Elizabeth A Yetley; Christine M Pfeiffer; Rosemary L Schleicher; Karen W Phinney; David A Lacher; Sylvia Christakos; John H Eckfeldt; James C Fleet; George Howard; Andrew N Hoofnagle; Siu L Hui; Gary L Lensmeyer; Joseph Massaro; Munro Peacock; Bernard Rosner; Donald Wiebe; Regan L Bailey; Paul M Coates; Anne C Looker; Christopher Sempos; Clifford L Johnson; Mary Frances Picciano Journal: J Nutr Date: 2010-09-29 Impact factor: 4.798
Authors: Ravinder J Singh; Robert L Taylor; G Satyanarayana Reddy; Stefan K G Grebe Journal: J Clin Endocrinol Metab Date: 2006-05-23 Impact factor: 5.958
Authors: Lesley A Inker; Christopher H Schmid; Hocine Tighiouart; John H Eckfeldt; Harold I Feldman; Tom Greene; John W Kusek; Jane Manzi; Frederick Van Lente; Yaping Lucy Zhang; Josef Coresh; Andrew S Levey Journal: N Engl J Med Date: 2012-07-05 Impact factor: 91.245
Authors: F Granado-Lorencio; I Blanco-Navarro; B Pérez-Sacristán; E Donoso-Navarro; R Silvestre-Mardomingo Journal: J Clin Endocrinol Metab Date: 2012-10-04 Impact factor: 5.958
Authors: Pamela L Lutsey; Mary R Rooney; Aaron R Folsom; Erin D Michos; Alvaro Alonso; Weihong Tang Journal: Vasc Med Date: 2018-02-05 Impact factor: 3.239
Authors: Rosemary L Schleicher; Maya R Sternberg; Anne C Looker; Elizabeth A Yetley; David A Lacher; Christopher T Sempos; Christine L Taylor; Ramon A Durazo-Arvizu; Khin L Maw; Madhulika Chaudhary-Webb; Clifford L Johnson; Christine M Pfeiffer Journal: J Nutr Date: 2016-04-06 Impact factor: 4.798
Authors: Casey M Rebholz; Morgan E Grams; Pamela L Lutsey; Andrew N Hoofnagle; Jeffrey R Misialek; Lesley A Inker; Andrew S Levey; Elizabeth Selvin; Chi-Yuan Hsu; Paul L Kimmel; Ramachandran S Vasan; John H Eckfeldt; Josef Coresh Journal: Am J Kidney Dis Date: 2015-10-23 Impact factor: 8.860
Authors: Andrea L C Schneider; Di Zhao; Pamela L Lutsey; Rebecca F Gottesman; A Richey Sharrett; Andreea M Rawlings; Alvaro Alonso; David Knopman; Thomas H Mosley; Elizabeth Selvin; Erin D Michos Journal: Neuroepidemiology Date: 2018-08-09 Impact factor: 3.282
Authors: Deborah M Mitchell; Kristin Ruppert; Nisha Udupa; Fatima Bassir; Karin Darakananda; Daniel H Solomon; Yinjuan Lian; Jane A Cauley; Arun S Karlamangla; Gail A Greendale; Joel S Finkelstein; Sherri-Ann M Burnett-Bowie Journal: Clin Endocrinol (Oxf) Date: 2019-04-24 Impact factor: 3.478
Authors: Ian R Rapson; Erin D Michos; Alvaro Alonso; Alan T Hirsch; Kunihiro Matsushita; Jared P Reis; Pamela L Lutsey Journal: Atherosclerosis Date: 2017-01-16 Impact factor: 5.162
Authors: Pamela L Lutsey; Christina M Parrinello; Jeffrey R Misialek; Andy N Hoofnagle; Clark M Henderson; Thomas J Laha; Erin D Michos; John H Eckfeldt; Elizabeth Selvin Journal: Clin Chem Date: 2016-09-30 Impact factor: 8.327
Authors: Rebeccah A McKibben; Di Zhao; Pamela L Lutsey; Andrea L C Schneider; Eliseo Guallar; Thomas H Mosley; Erin D Michos Journal: J Clin Endocrinol Metab Date: 2015-10-28 Impact factor: 5.958
Authors: Junichi Ishigami; Bernard G Jaar; Casey M Rebholz; Morgan E Grams; Erin D Michos; Myles Wolf; Csaba P Kovesdy; Shinichi Uchida; Josef Coresh; Pamela L Lutsey; Kunihiro Matsushita Journal: J Clin Endocrinol Metab Date: 2017-12-01 Impact factor: 5.958