| Literature DB >> 25577047 |
Carlo Ignazio Giovanni Tuberoso1, Francesca Congiu2, Gabriele Serreli2, Stefano Mameli2.
Abstract
Free amino acids (AA) and biogenic amines (BA) were quantified for the first time in Cannonau and Vermentino wines, the two most popular "Controlled Designation of Origin" wines from Sardinia (Italy). An analytical method for the simultaneous determination of AA and BA was developed, using selective derivatization with dansyl chloride followed by HPLC with fluorescence detection. Thirty-two compounds were identified in the wines analysed. High levels of AA were found, with proline being the most abundant with average levels of 1244 ± 398 and 1008 ± 281 mg/L in Cannonau and Vermentino wines, respectively. BA were detected at average concentrations <10mg/L, except putrescine which reached 20.5 ± 10.2mg/L in Cannonau wines. Histamine was never detected in any Vermentino wines. γ-Aminobutyric acid, 4-hydroxyproline, glycine, leucine+isoleucine and putrescine proved to be useful for differentiating Cannonau wines from Vermentino wines.Entities:
Keywords: Amino acids; Biogenic amines; Dansyl chloride; Fluorescence detection; HPLC; Validation; Wine
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Year: 2014 PMID： 25577047 DOI： 10.1016/j.foodchem.2014.11.120
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514