| Literature DB >> 2556854 |
D M Markovitz1, S Kenney, J Kamine, M S Smith, M Davis, E S Huang, C Rosen, J S Pagano.
Abstract
The BMLF1 region of the Epstein-Barr virus (EBV) genome and the immediate-early (IE) region of human cytomegalovirus (HCMV) both encode proteins which can trans-activate heterologous promoter/chloramphenicol acetyl transferase (CAT) constructs, including a human immunodeficiency virus type-1 promoter/CAT construct. We demonstrate here that this trans-activation by the EBV BMLF1 gene product, which we have previously shown to be largely post-transcriptional, is reporter gene dependent. In contrast, trans-activation by the HCMV-IE gene product(s), previously shown to be mediated at the RNA level, is seen regardless of whether CAT, human growth hormone, or beta-galactosidase is used as the reporter gene. Mutational analysis revealed no specific cis-acting sequences within the HIV-1 promoter which were required for trans-activation by the HCMV-IE gene product(s).Entities:
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Year: 1989 PMID： 2556854 DOI： 10.1016/0042-6822(89)90591-6
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616