Fatemeh Sadat Ghasemi1, Sima Rasti1, Ahmad Piroozmand2, Mojgan Bandehpour3,4, Bahram Kazemi3,4, Seyed Gholam Abbas Mousavi5, Amir Abdoli1,6. 1. a Department of Parasitology, Faculty of Medicine , Kashan University of Medical Sciences , Kashan , Iran . 2. b Department of Microbiology and Immunology, Faculty of Medicine , Kashan University of Medical Sciences , Kashan , Iran . 3. c Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences , Tehran , Iran . 4. d Department of Biotechnology, Faculty of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran . 5. e Department of Statistics and Public Health, Faculty of Health , Kashan University of Medical Sciences , Kashan , Iran , and. 6. f Department of Parasitology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
Abstract
OBJECTIVES: This study was aimed to evaluate the role of toxoplasmosis in etiology of abortion and stillbirth based on molecular and serological techniques. MATERIAL AND METHODS: A total of 110 pregnant women with abortion and stillbirth were enrolled as the case group, and 110 pregnant women with normal delivery were enrolled as the control group. Serological and molecular detections of Toxoplasma gondii were assessed by ELISA and PCR methods. RESULTS: The seroprevalence of IgG was 25.5% in the case group (26.8% in abortion and 21.4% in stillbirth) and 26.4% in the control group. IgM seropositivity was detected in 2.7% of the case group (3.6% in abortion and 0% in stillbirth) and 0.9% of the control group (p = 0.37). Toxoplasma gondii DNA was detected in 6.4% of the case group (7.3% in abortion and 3.6% in stillbirth) and 1.8% of the control group by PCR (p = 0.17). The major risk factor of congenital toxoplasmosis was the history of eating undercooked meat (p = 0.06). CONCLUSION: Results of this study revealed that the rate of PCR positive in women with abortion and stillbirth was 3.7 times higher than that in normal delivery, but the difference was not statistically significant. These findings suggest that toxoplasmosis can be involved in etiology of abortion and stillbirth.
OBJECTIVES: This study was aimed to evaluate the role of toxoplasmosis in etiology of abortion and stillbirth based on molecular and serological techniques. MATERIAL AND METHODS: A total of 110 pregnant women with abortion and stillbirth were enrolled as the case group, and 110 pregnant women with normal delivery were enrolled as the control group. Serological and molecular detections of Toxoplasma gondii were assessed by ELISA and PCR methods. RESULTS: The seroprevalence of IgG was 25.5% in the case group (26.8% in abortion and 21.4% in stillbirth) and 26.4% in the control group. IgM seropositivity was detected in 2.7% of the case group (3.6% in abortion and 0% in stillbirth) and 0.9% of the control group (p = 0.37). Toxoplasma gondii DNA was detected in 6.4% of the case group (7.3% in abortion and 3.6% in stillbirth) and 1.8% of the control group by PCR (p = 0.17). The major risk factor of congenital toxoplasmosis was the history of eating undercooked meat (p = 0.06). CONCLUSION: Results of this study revealed that the rate of PCR positive in women with abortion and stillbirth was 3.7 times higher than that in normal delivery, but the difference was not statistically significant. These findings suggest that toxoplasmosis can be involved in etiology of abortion and stillbirth.
Authors: C Alvarado-Esquivel; S J Pacheco-Vega; M Salcedo-Jaquez; L F Sánchez-Anguiano; J Hernández-Tinoco; E Rábago-Sánchez; M M Centeno-Tinoco; I D Flores-Garcia; A Ramos-Nevarez; S M Cerrillo-Soto; C A Guido-Arreola; I Beristain-García; O Liesenfeld; L O Berumen-Segovia; L Saenz-Soto; A Sifuentes-Álvarez Journal: Eur J Microbiol Immunol (Bp) Date: 2015-06-18
Authors: Illuminata Machumi; Mariam M Mirambo; Deodatus Ruganuza; Peter Rambau; Anthony N Massinde; Albert Kihunrwa; Stephen E Mshana; Domenica Morona Journal: East Afr Health Res J Date: 2017-07-01