Jinming Duan1, Hongda Fang2, Bing Su3, Jinfang Chen2, Jinmei Lin2. 1. Research Center of Water Treatment Engineering, Jimei University, Xiamen 361021, PR China; School of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China. Electronic address: duanjinming@jmu.edu.cn. 2. Research Center of Water Treatment Engineering, Jimei University, Xiamen 361021, PR China; School of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China. 3. School of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China.
Abstract
A novel halophilic bacterium capable of heterotrophic nitrification-aerobic denitrification was isolated from marine sediments and identified as Vibrio diabolicus SF16. It had ability to remove 91.82% of NH4(+)-N (119.77 mg/L) and 99.71% of NO3(-)-N (136.43 mg/L). The nitrogen balance showed that 35.83% of initial NH4(+)-N (119.77 mg/L) was changed to intracellular nitrogen, and 53.98% of the initial NH4(+)-N was converted to gaseous denitrification products. The existence of napA gene further proved the aerobic denitrification ability of strain SF16. The optimum culture conditions were salinity 1-5%, sodium acetate as carbon source, C/N 10, and pH 7.5-9.5. When an aerated biological filter system inoculated with strain SF16 was employed to treat saline wastewater, the average removal efficiency of NH4(+)-N and TN reached 97.14% and 73.92%, respectively, indicating great potential of strain SF16 for future full-scale applications.
A novel halophilic bacterium capable of heterotrophic nitrification-aerobic denitrification was isolated from marine sediments and identified as Vibrio diabolicus SF16. It had ability to remove 91.82% of n class="Chemical">NH4(+)-N (119.77 mg/L) and 99.71% of NO3(-)-N (136.43 mg/L). The nitrogen balance showed that 35.83% of initial NH4(+)-N (119.77 mg/L) was changed to intracellular nitrogen, and 53.98% of the initial NH4(+)-N was converted to gaseous denitrification products. The existence of napA gene further proved the aerobic denitrification ability of strain SF16. The optimum culture conditions were salinity 1-5%, sodium acetate as carbon source, C/N 10, and pH 7.5-9.5. When an aerated biological filter system inoculated with strain SF16 was employed to treat saline wastewater, the average removal efficiency of NH4(+)-N and TN reached 97.14% and 73.92%, respectively, indicating great potential of strain SF16 for future full-scale applications.
Authors: Zixia Qiao; Yaoguo Wu; Jin Qian; Sihai Hu; Jiangwei Chan; Xiaoyan Liu; Ran Sun; Wendong Wang; Bo Zhou Journal: Environ Sci Pollut Res Int Date: 2020-01-08 Impact factor: 4.223