Mingzhang Guo1, Shuo Ding2, Changhui Zhao3, Xinxi Gu4, Xiaoyun He1, Kunlun Huang1, Yunbo Luo1, Zhihong Liang1, Hongtao Tian5, Wentao Xu6. 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China; College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, PR China. 3. Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA. 4. College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, PR China. 5. College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, PR China. Electronic address: tht631022@163.com. 6. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address: xuwentao@cau.edu.cn.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Many Chinese herbs are traditionally used as medicine to improve the functions of gastrointestinal tract. Some of these herbs are also promising agents for the improvement of the gut microbiota and the treatment of ulcerative colitis. MATERIALS AND METHODS: By screening seven traditional Chinese herbs, we found that Red Ginseng and Semen Coicis were the most effective in promoting the growth of probiotics including Lactobacillus and Bifidobacterium in vitro. We then evaluated the effects of Red Ginseng and Semen Coicis on the growth of the bacterial pathogens (Escherichia coli, Staphylococcus aureus, and Salmonella spp.) in vitro. In in vivo experiment, we gavage administrated trinitro-benzene-sulfonic acid induced ulcerative colitis (UC) rats with Red Ginseng and Semen Coicis extracts. After two weeks treatment, we analyzed the structure of the gut microbiota and examined the UC symptoms by employing qPCR and animal pathology detection techniques. RESULTS: Both Red Ginseng and Semen Coicis promoted the growth of probiotics - Bifidobacterium and Lactobacillus in vitro. Red Ginseng also inhibited the growth of some pathogen strains. In vivo, Red Ginseng and Semen Coicis improved the structure of gut microbiota and relieved the symptoms of ulcerative colitis in vivo. Compared with Semen Coicis, Red Ginseng was more effective in relieving the symptoms of ulcerative colitis. CONCLUSIONS: Red Ginseng could promote the growth of probiotic bacteria in vitro. Red Ginseng and, to a lesser extent Semen Coicis, gave positive results in an experimental in vivo model for ulcerative colitis.
ETHNOPHARMACOLOGICAL RELEVANCE: Many Chinese herbs are traditionally used as medicine to improve the functions of gastrointestinal tract. Some of these herbs are also promising agents for the improvement of the gut microbiota and the treatment of ulcerative colitis. MATERIALS AND METHODS: By screening seven traditional Chinese herbs, we found that Red Ginseng and Semen Coicis were the most effective in promoting the growth of probiotics including Lactobacillus and Bifidobacterium in vitro. We then evaluated the effects of Red Ginseng and Semen Coicis on the growth of the bacterial pathogens (Escherichia coli, Staphylococcus aureus, and Salmonella spp.) in vitro. In in vivo experiment, we gavage administrated trinitro-benzene-sulfonic acid induced ulcerative colitis (UC) rats with Red Ginseng and Semen Coicis extracts. After two weeks treatment, we analyzed the structure of the gut microbiota and examined the UC symptoms by employing qPCR and animal pathology detection techniques. RESULTS: Both Red Ginseng and Semen Coicis promoted the growth of probiotics - Bifidobacterium and Lactobacillus in vitro. Red Ginseng also inhibited the growth of some pathogen strains. In vivo, Red Ginseng and Semen Coicis improved the structure of gut microbiota and relieved the symptoms of ulcerative colitis in vivo. Compared with Semen Coicis, Red Ginseng was more effective in relieving the symptoms of ulcerative colitis. CONCLUSIONS: Red Ginseng could promote the growth of probiotic bacteria in vitro. Red Ginseng and, to a lesser extent Semen Coicis, gave positive results in an experimental in vivo model for ulcerative colitis.
Authors: Andreea Zurbau; Lea Smircic Duvnjak; Sasa Magas; Elena Jovanovski; Jelena Miocic; Alexandra L Jenkins; David J A Jenkins; Robert G Josse; Lawrence A Leiter; John L Sievenpiper; Vladimir Vuksan Journal: Eur J Nutr Date: 2021-01-24 Impact factor: 5.614
Authors: Hayley T Morris; Loic Fort; Heather J Spence; Rachana Patel; David F Vincent; James H Park; Scott B Snapper; Francis A Carey; Owen J Sansom; Laura M Machesky Journal: J Pathol Date: 2018-05-28 Impact factor: 7.996