Literature DB >> 2555407

A microtiter plate assay for inorganic phosphate.

M Hoenig1, R J Lee, D C Ferguson.   

Abstract

A microtiter assay for the detection of picomolar quantities of inorganic phosphate has been described. The assay, linear between 50 and 1000 pmol of inorganic phosphate, is simple and rapid, with results obtainable in several minutes. Results from 5'-nucleotidase and (Ca2+ + Mg2+)ATPase assays using this method were compared with conventional phosphate assays and showed a high degree of correlation. The high sensitivity of this assay and the small sample size needed allows its widespread use in biochemical studies involving the generation of inorganic phosphate.

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Year:  1989        PMID： 2555407     DOI： 10.1016/0165-022x(89)90031-6

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  18 in total

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Authors:  Huanchen Wang; Vasudha S Nair; Ashley A Holland; Samanta Capolicchio; Henning J Jessen; Michael K Johnson; Stephen B Shears
Journal:  Biochemistry       Date:  2015-10-09       Impact factor: 3.162

2.  Gramicidin A directly inhibits mammalian Na(+)/K (+)-ATPase.

Authors:  Yohei Takada; Kentaro Matsuo; Takao Kataoka
Journal:  Mol Cell Biochem       Date:  2008-07-13       Impact factor: 3.396

3.  MinC protein shortens FtsZ protofilaments by preferentially interacting with GDP-bound subunits.

Authors:  Víctor M Hernández-Rocamora; Concepción García-Montañés; Belén Reija; Begoña Monterroso; William Margolin; Carlos Alfonso; Silvia Zorrilla; Germán Rivas
Journal:  J Biol Chem       Date:  2013-07-12       Impact factor: 5.157

4.  Expression of functional Plasmodium falciparum enzymes using a wheat germ cell-free system.

Authors:  Devaraja G Mudeppa; Pradipsinh K Rathod
Journal:  Eukaryot Cell       Date:  2013-10-11

5.  Receptor-dependent compartmentalization of PPIP5K1, a kinase with a cryptic polyphosphoinositide binding domain.

Authors:  Nikhil A Gokhale; Angelika Zaremba; Stephen B Shears
Journal:  Biochem J       Date:  2011-03-15       Impact factor: 3.857

6.  Paralogous murine Nudt10 and Nudt11 genes have differential expression patterns but encode identical proteins that are physiologically competent diphosphoinositol polyphosphate phosphohydrolases.

Authors:  Len V Hua; Kiyoshi Hidaka; Xavier Pesesse; Larry D Barnes; Stephen B Shears
Journal:  Biochem J       Date:  2003-07-01       Impact factor: 3.857

7.  Evidence That Bacteriophage λ Kil Peptide Inhibits Bacterial Cell Division by Disrupting FtsZ Protofilaments and Sequestering Protein Subunits.

Authors:  Víctor M Hernández-Rocamora; Carlos Alfonso; William Margolin; Silvia Zorrilla; Germán Rivas
Journal:  J Biol Chem       Date:  2015-06-29       Impact factor: 5.157

8.  The Significance of the Bifunctional Kinase/Phosphatase Activities of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for Coupling Inositol Pyrophosphate Cell Signaling to Cellular Phosphate Homeostasis.

Authors:  Chunfang Gu; Hoai-Nghia Nguyen; Alexandre Hofer; Henning J Jessen; Xuming Dai; Huanchen Wang; Stephen B Shears
Journal:  J Biol Chem       Date:  2017-01-26       Impact factor: 5.157

9.  Structural and biochemical characterization of Siw14: A protein-tyrosine phosphatase fold that metabolizes inositol pyrophosphates.

Authors:  Huanchen Wang; Chunfang Gu; Ronda J Rolfes; Henning J Jessen; Stephen B Shears
Journal:  J Biol Chem       Date:  2018-03-14       Impact factor: 5.157

10.  A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase.

Authors:  S T Safrany; J J Caffrey; X Yang; M E Bembenek; M B Moyer; W A Burkhart; S B Shears
Journal:  EMBO J       Date:  1998-11-16       Impact factor: 11.598
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