Literature DB >> 25552360

Cost-effective pooling of DNA from nasopharyngeal swab samples for large-scale detection of bacteria by real-time PCR.

Sophie Edouard1, Elsa Prudent1, Philippe Gautret1, Ziad A Memish2, Didier Raoult3.   

Abstract

We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2014        PMID： 25552360      PMCID： PMC4390636          DOI： 10.1128/JCM.03609-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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