Yongbo Zhao1, Guangxing Feng1, Yanzhi Wang1, Yuehong Yue2, Weichao Zhao1. 1. Department of Cardiovascular Surgery, Fourth Hospital of Hebei Medical University Shijiazhuang, China. 2. Department of Neurology, Hebei General Hospital Shijiazhuang, China.
Abstract
OBJECTIVE: Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as transcriptional regulation, cell growth and tumorigenesis. However, little was known about whether lncRNA HIF 1 alpha-antisense RNA 1 (HIF1a-AS1) in regulating the proliferation and apoptosis of VSMCs in vitro and the expression of HIF1a-AS1 in serum of TAA patients. METHODS: The cell viability was detected by the CCK8 assay. The cell apoptosis was assessed by annexin V-PI double-labeling staining. Expression of genes and proteins were analyzed by real-time PCR and western blotting respectively. Cells were transfected with siRNAs as a gene silencing methods. RESULTS: In serum of TAA patients, the expression of HIF1a-AS1 was significantly increased (superior to 6 folds) compared to the normal control. Moreover, PA induced cell apoptosis in VSMCs in a time- and dose-dependent manner, and the proportion of the apoptotic cells had gained as compared to untreatment group. PA also induced upregulation expression of HIF1a-AS1. We also found that transfection of cells with HIF1a-AS1 siRNA decreased the expression of caspase3 and caspase8 and increased the expression of Bcl2, and protected PA-induced cell apoptosis in VSMCs. CONCLUSIONS: HIF1a-AS1 was overexpressed in the thoracoabdominal aorta aneurysm and the interaction between HIF1a-AS1 and apoptotic proteins plays a key role in the proliferation and apoptosis of VSMCs in vitro, which may contribute to the pathogenesis of thoracoabdominal aorta aneurysm.
OBJECTIVE: Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as transcriptional regulation, cell growth and tumorigenesis. However, little was known about whether lncRNA HIF 1 alpha-antisense RNA 1 (HIF1a-AS1) in regulating the proliferation and apoptosis of VSMCs in vitro and the expression of HIF1a-AS1 in serum of TAA patients. METHODS: The cell viability was detected by the CCK8 assay. The cell apoptosis was assessed by annexin V-PI double-labeling staining. Expression of genes and proteins were analyzed by real-time PCR and western blotting respectively. Cells were transfected with siRNAs as a gene silencing methods. RESULTS: In serum of TAA patients, the expression of HIF1a-AS1 was significantly increased (superior to 6 folds) compared to the normal control. Moreover, PA induced cell apoptosis in VSMCs in a time- and dose-dependent manner, and the proportion of the apoptotic cells had gained as compared to untreatment group. PA also induced upregulation expression of HIF1a-AS1. We also found that transfection of cells with HIF1a-AS1 siRNA decreased the expression of caspase3 and caspase8 and increased the expression of Bcl2, and protected PA-induced cell apoptosis in VSMCs. CONCLUSIONS:HIF1a-AS1 was overexpressed in the thoracoabdominal aorta aneurysm and the interaction between HIF1a-AS1 and apoptotic proteins plays a key role in the proliferation and apoptosis of VSMCs in vitro, which may contribute to the pathogenesis of thoracoabdominal aorta aneurysm.
Authors: Khelifa Arab; Yoon Jung Park; Anders M Lindroth; Andrea Schäfer; Christopher Oakes; Dieter Weichenhan; Annekatrin Lukanova; Eva Lundin; Angela Risch; Michael Meister; Hendrik Dienemann; Gerhard Dyckhoff; Christel Herold-Mende; Ingrid Grummt; Christof Niehrs; Christoph Plass Journal: Mol Cell Date: 2014-07-31 Impact factor: 17.970
Authors: Stefanie S Portelli; Elizabeth N Robertson; Cassandra Malecki; Kiersten A Liddy; Brett D Hambly; Richmond W Jeremy Journal: Biophys Rev Date: 2018-09-28
Authors: Laura-Eve Mantella; Krishna K Singh; Paul Sandhu; Crystal Kantores; Azza Ramadan; Nadiya Khyzha; Adrian Quan; Mohammed Al-Omran; Jason E Fish; Robert P Jankov; Subodh Verma Journal: Mol Cell Biochem Date: 2017-05-19 Impact factor: 3.396
Authors: B Paul Herring; April M Hoggatt; Sarah L Griffith; Jeanette N McClintick; Patricia J Gallagher Journal: Physiol Genomics Date: 2016-12-30 Impact factor: 3.107