Jin-Ok Ahn1, Ye-Rin Coh1, Hee-Woo Lee1, Il-Seob Shin2, Sung-Keun Kang2, Hwa-Young Youn3. 1. Department of Veterinary Internal Medicine and Research Insititute for Veterinary Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea. 2. Stem Cell Research Center, K-STEMCELL Co. Ltd., Seoul, Republic of Korea. 3. Department of Veterinary Internal Medicine and Research Insititute for Veterinary Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea hyyoun@snu.ac.kr.
Abstract
BACKGROUND/AIM: The effects of adipose tissue-derived mesenchymal stem cells (AT-MSCs) on the growth of human malignancies, including melanoma, are controversial and the underlying mechanisms are not yet-well understood. The aim of the present study was to investigate the in vitro and in vivo anti-tumor effects of human AT-MSCs on human melanoma. MATERIALS AND METHODS: The inhibitory effect of AT-MSC-conditioned medium (AT-MSC-CM) on the growth of A375SM and A375P (human melanoma) cells was evaluated using a cell viability assay. Cell-cycle arrest and apoptosis in melanoma cells were investigated by flow cytometry and western blot analysis. To evaluate the in vivo anti-tumor effect of AT-MSCs, CM-DiI-labeled AT-MSCs were circumtumorally injected in tumor-bearing athymic mice and tumor size was measured. RESULTS: AT-MSC-CM inhibited melanoma growth by altering cell-cycle distribution and inducing apoptosis in vitro. AT-MSCs suppressed tumor growth in tumor-bearing athymic mice and fluorescence analysis showed that AT-MSCs migrated efficiently to tumor tissues. CONCLUSION: AT-MSCs inhibit the growth of melanoma suggesting promise as a novel therapeutic agent for melanoma. Copyright
BACKGROUND/AIM: The effects of adipose tissue-derived mesenchymal stem cells (AT-MSCs) on the growth of humanmalignancies, including melanoma, are controversial and the underlying mechanisms are not yet-well understood. The aim of the present study was to investigate the in vitro and in vivo anti-tumor effects of human AT-MSCs on humanmelanoma. MATERIALS AND METHODS: The inhibitory effect of AT-MSC-conditioned medium (AT-MSC-CM) on the growth of A375SM and A375P (humanmelanoma) cells was evaluated using a cell viability assay. Cell-cycle arrest and apoptosis in melanoma cells were investigated by flow cytometry and western blot analysis. To evaluate the in vivo anti-tumor effect of AT-MSCs, CM-DiI-labeled AT-MSCs were circumtumorally injected in tumor-bearing athymic mice and tumor size was measured. RESULTS: AT-MSC-CM inhibited melanoma growth by altering cell-cycle distribution and inducing apoptosis in vitro. AT-MSCs suppressed tumor growth in tumor-bearing athymic mice and fluorescence analysis showed that AT-MSCs migrated efficiently to tumor tissues. CONCLUSION: AT-MSCs inhibit the growth of melanoma suggesting promise as a novel therapeutic agent for melanoma. Copyright
Authors: Cheng Zhang; Shi-Jie Yang; Qin Wen; Jiang F Zhong; Xue-Lian Chen; Andres Stucky; Michael F Press; Xi Zhang Journal: J Cancer Date: 2017-01-01 Impact factor: 4.207