Xiangming Ji1, Anee Usman1, Nurul H Razalli1, Ravigadevi Sambanthamurthi2, Smiti V Gupta3. 1. Department of Nutrition and Food Science, Wayne State University, Detroit, MI, U.S.A. 2. Malaysian Palm Oil Board, Bandar Baru Bangi, Selangor, Malaysia. 3. Department of Nutrition and Food Science, Wayne State University, Detroit, MI, U.S.A sgupta@wayne.edu.
Abstract
BACKGROUND: Oil palm phenolics (OPP) or Palm Juice (PJ), a water soluble extract from the palm fruit (Elaies guineensis) has been documented to have anti-carcinogenic activities in various cancer types. MATERIALS AND METHODS: To investigate OPP effects in pancreatic cancer (PaCa) cells, two PaCa cell lines (PANC-1 and BxPC-3) were treated with different OPP doses. The anti-proliferative, apoptotic and anti-invasive properties of OPP were evaluated using MTS, cytoplasmic histone-DNA fragmentation and matrigel invasive assays, respectively. RESULTS: OPP suppressed PaCa proliferation in a dose-dependent manner. Its anti-invasive effects were validated by decreased expressions of MMP-9 and VEGF. Cell-cycle analysis demonstrated that cells were arrested in the S phase. OPP-induced apoptosis was associated with decrease in survivin and Bcl-XL expressions and increased expression of cleaved caspase-3, caspase-9 and PARP. CONCLUSION: Overall, our results demonstrate the anti-tumor effects of OPP on PaCa cells, providing initial evidence towards its potential therapeutic use. Copyright
BACKGROUND:Oil palm phenolics (OPP) or Palm Juice (PJ), a water soluble extract from the palm fruit (Elaies guineensis) has been documented to have anti-carcinogenic activities in various cancer types. MATERIALS AND METHODS: To investigate OPP effects in pancreatic cancer (PaCa) cells, two PaCa cell lines (PANC-1 and BxPC-3) were treated with different OPP doses. The anti-proliferative, apoptotic and anti-invasive properties of OPP were evaluated using MTS, cytoplasmic histone-DNA fragmentation and matrigel invasive assays, respectively. RESULTS:OPP suppressed PaCa proliferation in a dose-dependent manner. Its anti-invasive effects were validated by decreased expressions of MMP-9 and VEGF. Cell-cycle analysis demonstrated that cells were arrested in the S phase. OPP-induced apoptosis was associated with decrease in survivin and Bcl-XL expressions and increased expression of cleaved caspase-3, caspase-9 and PARP. CONCLUSION: Overall, our results demonstrate the anti-tumor effects of OPP on PaCa cells, providing initial evidence towards its potential therapeutic use. Copyright
Authors: Robert P Weinberg; Vera V Koledova; Kirsten Schneider; T G Sambandan; Adlai Grayson; Gal Zeidman; Anastasia Artamonova; Ravigadevi Sambanthamurthi; Syed Fairus; Anthony J Sinskey; ChoKyun Rha Journal: Sci Rep Date: 2018-11-06 Impact factor: 4.379
Authors: Robert P Weinberg; Vera V Koledova; Avinaash Subramaniam; Kirsten Schneider; Anastasia Artamonova; Ravigadevi Sambanthamurthi; K C Hayes; Anthony J Sinskey; ChoKyun Rha Journal: Sci Rep Date: 2019-12-09 Impact factor: 4.379