Literature DB >> 2554888

Potassium channels in the luminal membrane of rabbit proximal straight tubule. Evidence from vesicle studies.

C Jacobsen1, H Røigaard-Petersen, M I Sheikh.   

Abstract

The characteristics of 86Rb+ fluxes through K+ channels in luminal-membrane vesicles isolated from the pars recta of rabbit proximal tubule were studied. In KCl-loaded vesicles from the pars recta, transient accumulation of 86Rb+ is observed which is modestly inhibited by BaCl2 and blocked by CdCl2. The isotope accumulation is driven by an electrical diffusion potential, as shown in experiments using either these membrane vesicles loaded with different anions, or an outwardly directed Li+ gradient with a Li+ ionophore. The vesicles containing the channel show a cation selectivity with the order K+ greater than Rb+ greater than choline+ greater than or equal to Li+ greater than Na+. The CdCl2-sensitive 86Rb+ flux is dependent on intravesicular Ca2+. Increasing concentrations of Ca2+ gradually decreased the 86Rb+ uptake and at 1 microM-Ca2+ the CdCl2-sensitive isotope flux is nearly abolished.

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Year:  1989        PMID： 2554888      PMCID： PMC1133257          DOI： 10.1042/bj2620271

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

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Authors:  J C SKOU
Journal:  Physiol Rev       Date:  1965-07       Impact factor: 37.312

2.  The application of patch clamp methods to ocular epithelia.

Authors:  J L Rae
Journal:  Curr Eye Res       Date:  1985-04       Impact factor: 2.424

3.  A simple and sensitive procedure for measuring isotope fluxes through ion-specific channels in heterogenous populations of membrane vesicles.

Authors:  H Garty; B Rudy; S J Karlish
Journal:  J Biol Chem       Date:  1983-11-10       Impact factor: 5.157

Review 4.  Calcium-activated potassium channels and their role in secretion.

Authors:  O H Petersen; Y Maruyama
Journal:  Nature       Date:  1984 Feb 23-29       Impact factor: 49.962

5.  Cl- - and K+-related fluctuations of ionic current through oxyntic cells in frog gastric mucosa.

Authors:  W Zeiske; T E Machen; W Van Driessche
Journal:  Am J Physiol       Date:  1983-12

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Authors:  E G Schneider; B Sacktor
Journal:  J Biol Chem       Date:  1980-08-25       Impact factor: 5.157

7.  A simplification of the protein assay method of Lowry et al. which is more generally applicable.

Authors:  G L Peterson
Journal:  Anal Biochem       Date:  1977-12       Impact factor: 3.365

8.  Passive rubidium fluxes mediated by Na-K-ATPase reconstituted into phospholipid vesicles when ATP- and phosphate-free.

Authors:  S J Karlish; W D Stein
Journal:  J Physiol       Date:  1982-07       Impact factor: 5.182

9.  Noise analysis reveals K+ channel conductance fluctuations in the apical membrane of rabbit colon.

Authors:  N K Wills; W Zeiske; W Van Driessche
Journal:  J Membr Biol       Date:  1982       Impact factor: 1.843

10.  An efficient method for the isolation and separation of basolateral-membrane and luminal-membrane vesicles from rabbit kidney cortex.

Authors:  M I Sheikh; U Kragh-Hansen; K E Jørgensen; H Røigaard-Petersen
Journal:  Biochem J       Date:  1982-11-15       Impact factor: 3.857
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  1 in total

1.  Non-selective cation channels in basolateral-membrane vesicles from pars recta of rabbit kidney proximal tubule.

Authors:  J Blokkebak-Poulsen; M I Sheikh; C Jacobsen
Journal:  Biochem J       Date:  1990-12-15       Impact factor: 3.857
  1 in total

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