Literature DB >> 2554859

A strategy simplifying site-directed mutagenesis in the CD4-binding region of HIV gp 120.

A Hemming1, L Lundberg, S Olofsson.   

Abstract

By site-directed mutagenesis, two unique restriction sites, surrounding the CD4-binding DNA region of HIV gp 120, were created. The mutations do not cause amino acid substitutions but generate a DNA fragment, which is stable when cloned into M 13 for further mutagenesis and is also easy to reclone.

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Year:  1989        PMID： 2554859     DOI： 10.1007/BF01317925

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  8 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Coexpression of human immunodeficiency virus envelope proteins and tat from a single simian virus 40 late replacement vector.

Authors:  D Rekosh; A Nygren; P Flodby; M L Hammarskjöld; H Wigzell
Journal:  Proc Natl Acad Sci U S A       Date:  1988-01       Impact factor: 11.205

3.  95- and 25-kDa fragments of the human immunodeficiency virus envelope glycoprotein gp120 bind to the CD4 receptor.

Authors:  A Nygren; T Bergman; T Matthews; H Jörnvall; H Wigzell
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

4.  Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis.

Authors:  K L Nakamaye; F Eckstein
Journal:  Nucleic Acids Res       Date:  1986-12-22       Impact factor: 16.971

5.  Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptor.

Authors:  L A Lasky; G Nakamura; D H Smith; C Fennie; C Shimasaki; E Patzer; P Berman; T Gregory; D J Capon
Journal:  Cell       Date:  1987-09-11       Impact factor: 41.582

6.  Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1.

Authors:  M Kowalski; J Potz; L Basiripour; T Dorfman; W C Goh; E Terwilliger; A Dayton; C Rosen; W Haseltine; J Sodroski
Journal:  Science       Date:  1987-09-11       Impact factor: 47.728

7.  Improved oligonucleotide site-directed mutagenesis using M13 vectors.

Authors:  P Carter; H Bedouelle; G Winter
Journal:  Nucleic Acids Res       Date:  1985-06-25       Impact factor: 16.971

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
  8 in total
  4 in total

1.  Protein engineering and site-directed mutagenesis. Patents and literature.

Authors:  J S Dordick
Journal:  Appl Biochem Biotechnol       Date:  1990-10       Impact factor: 2.926

2.  Conserved N-linked oligosaccharides of the C-terminal portion of human immunodeficiency virus type 1 gp120 and viral susceptibility to neutralizing antibodies.

Authors:  A Hemming; G J Gram; A Bolmstedt; B Losman; J E Hansen; A Ricksten; S Olofsson
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

3.  Identification of three N-linked glycans in the V4-V5 region of HIV-1 gp 120, dispensable for CD4-binding and fusion activity of gp 120.

Authors:  A Hemming; A Bolmstedt; B Jansson; J E Hansen; B Travis; S L Hu; S Olofsson
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

4.  Identification of an N-linked glycan in the V1-loop of HIV-1 gp120 influencing neutralization by anti-V3 antibodies and soluble CD4.

Authors:  G J Gram; A Hemming; A Bolmstedt; B Jansson; S Olofsson; L Akerblom; J O Nielsen; J E Hansen
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574
  4 in total

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