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Literature DB >> 25548277

Inhibition of transforming growth factor β (TGF-β) signaling can substitute for Oct4 protein in reprogramming and maintain pluripotency.

Fangzhi Tan¹, Cheng Qian¹, Ke Tang², Saber Mohamed Abd-Allah³, Naihe Jing⁴.

Abstract

Mouse pluripotent stem cells (PSCs), such as ES cells and induced PSCs (iPSCs), are an excellent system to investigate the molecular and cellular mechanisms involved in early embryonic development. The signaling pathways orchestrated by leukemia inhibitor factor/STAT3, Wnt/β-catenin, and FGF/MEK/ERK play key roles in the generation of pluripotency. However, the function of TGF-β signaling in this process remains elusive. Here we show that inhibiting TGF-β signaling with its inhibitor SB431542 can substitute for Oct4 during reprogramming. Moreover, inhibiting TGF-β signaling can sustain the pluripotency of iPSCs and ES cells through modulating FGF/MEK/ERK signaling. Therefore, this study reveals a novel function of TGF-β signaling inhibition in the generation and maintenance of PSCs.

Entities: Chemical Disease Gene Species

Keywords: ERK; ES Cell; Induced Pluripotent Stem Cell (iPSC); Oct4; Reprogramming; TGF-β

Mesh：

Substances：

Year: 2014 PMID： 25548277 PMCID： PMC4326853 DOI： 10.1074/jbc.M114.609016

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

52 in total

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7. Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling.

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