Literature DB >> 2554749

Immunoprecipitation of labeled antigens with Eupergit C1Z.

S Grässel1, A Röling, A Hasilik.   

Abstract

Eupergit C1Z, a nonporous sedimentable acrylic polymer is shown to be a convenient solid support for isolation of antigens with high purity from small amounts of detergent-solubilized cells. Several applications of Eupergit C1Z coupled with antibodies and anti-antibodies are described, and compared with two other methods of immunoprecipitation.

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Year:  1989        PMID： 2554749     DOI： 10.1016/0003-2697(89)90089-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

1.  Expression and maturation of human cathepsin D in baby-hamster kidney cells.

Authors:  M Horst; A Hasilik
Journal:  Biochem J       Date:  1991-01-15       Impact factor: 3.857

2.  Sphingomyelin-enriched microdomains at the Golgi complex.

Authors:  I Gkantiragas; B Brügger; E Stüven; D Kaloyanova; X Y Li; K Löhr; F Lottspeich; F T Wieland; J B Helms
Journal:  Mol Biol Cell       Date:  2001-06       Impact factor: 4.138

3.  Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins.

Authors:  P Angelisová; I Hilgert; V Horejsí
Journal:  Immunogenetics       Date:  1994       Impact factor: 2.846

4.  Engagement of T cell receptor triggers its recruitment to low-density detergent-insoluble membrane domains.

Authors:  C Montixi; C Langlet; A M Bernard; J Thimonier; C Dubois; M A Wurbel; J P Chauvin; M Pierres; H T He
Journal:  EMBO J       Date:  1998-09-15       Impact factor: 11.598

5.  An alternative way of CD4 and CD8 association with protein kinases of the Src family.

Authors:  T Cinek; I Hilgert; V Horejsí
Journal:  Immunogenetics       Date:  1995       Impact factor: 2.846

6.  Distinctive inhibition of the lysosomal targeting of lysozyme and cathepsin D by drugs affecting pH gradients and protein kinase C.

Authors:  J Radons; U Biewusch; S Grässel; H J Geuze; A Hasilik
Journal:  Biochem J       Date:  1994-09-01       Impact factor: 3.857

7.  Immunocytochemical localisation of some lysosomal hydrolases, their presence in luminal fluid and their directional secretion by human epididymal cells in culture.

Authors:  S Raczek; C H Yeung; A Hasilik; H Robenek; L Hertle; H Schulze; T G Cooper
Journal:  Cell Tissue Res       Date:  1995-05       Impact factor: 5.249

8.  Differential segregation of human and hamster cathepsin D in transfected baby-hamster kidney cells.

Authors:  C Isidoro; M Horst; F M Baccino; A Hasilik
Journal:  Biochem J       Date:  1991-01-15       Impact factor: 3.857

9.  Sorting of synaptophysin into special vesicles in nonneuroendocrine epithelial cells.

Authors:  R E Leube; U Leimer; C Grund; W W Franke; N Harth; B Wiedenmann
Journal:  J Cell Biol       Date:  1994-12       Impact factor: 10.539
  9 in total

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