Neda Ghaffari1, Samuel Parry2, Michal A Elovitz2, Celeste P Durnwald2. 1. Department of Obstetrics and Gynecology, Maternal and Child Health Research Program, Center for Research on Reproduction and Women's Health, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, United States neda.ghaffari@uphs.upenn.edu. 2. Department of Obstetrics and Gynecology, Maternal and Child Health Research Program, Center for Research on Reproduction and Women's Health, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, United States.
Abstract
OBJECTIVE: Exposure to maternal obesity in utero predisposes offspring to obesity and metabolic disease. This study investigated whether maternal obesity is associated with alterations in expression of fetal microRNA (miRNA). STUDY DESIGN: A cohort study of women with body mass index (BMI) ≥35 kg/m(2) (n = 16) versus those with normal BMI 20 to 24.9 (n = 20) was performed. All participants had normal glucose tolerance (1-hour glucose challenge test <130) and normally grown neonates (2700-3500 g). Umbilical cord samples were collected immediately after delivery. Expression of miRNA was assessed using Affymetrix GeneChip miRNA 3.0 Arrays. Differential miRNA expression was determined using Student t tests with Benjamini-Hocherg correction. RESULTS: For 1733 human mature miRNAs, the expression levels were not statistically different in umbilical cord blood samples from pregnancies of obese women compared to controls. CONCLUSION: Expression of fetal miRNA is not altered in umbilical cord blood in response to in utero exposure to obesity. Alternate mechanisms underlying the fetal effects of maternal obesity should be explored.
OBJECTIVE: Exposure to maternal obesity in utero predisposes offspring to obesity and metabolic disease. This study investigated whether maternal obesity is associated with alterations in expression of fetal microRNA (miRNA). STUDY DESIGN: A cohort study of women with body mass index (BMI) ≥35 kg/m(2) (n = 16) versus those with normal BMI 20 to 24.9 (n = 20) was performed. All participants had normal glucose tolerance (1-hour glucose challenge test <130) and normally grown neonates (2700-3500 g). Umbilical cord samples were collected immediately after delivery. Expression of miRNA was assessed using Affymetrix GeneChip miRNA 3.0 Arrays. Differential miRNA expression was determined using Student t tests with Benjamini-Hocherg correction. RESULTS: For 1733 human mature miRNAs, the expression levels were not statistically different in umbilical cord blood samples from pregnancies of obesewomen compared to controls. CONCLUSION: Expression of fetal miRNA is not altered in umbilical cord blood in response to in utero exposure to obesity. Alternate mechanisms underlying the fetal effects of maternal obesity should be explored.
Authors: Anna Zampetaki; Stefan Kiechl; Ignat Drozdov; Peter Willeit; Ursula Mayr; Marianna Prokopi; Agnes Mayr; Siegfried Weger; Friedrich Oberhollenzer; Enzo Bonora; Ajay Shah; Johann Willeit; Manuel Mayr Journal: Circ Res Date: 2010-07-22 Impact factor: 17.367
Authors: Xiaozhong Wen; Elizabeth W Triche; Joseph W Hogan; Edmond D Shenassa; Stephen L Buka Journal: Pediatrics Date: 2011-02-07 Impact factor: 7.124
Authors: D Montenegro; R Romero; S S Kim; A L Tarca; S Draghici; J P Kusanovic; J S Kim; D C Lee; O Erez; F Gotsch; S S Hassan; C J Kim Journal: J Pathol Date: 2009-01 Impact factor: 7.996
Authors: Alina Maloyan; Sribalasubashini Muralimanoharan; Steven Huffman; Laura A Cox; Peter W Nathanielsz; Leslie Myatt; Mark J Nijland Journal: Physiol Genomics Date: 2013-08-06 Impact factor: 3.107
Authors: Keith M Godfrey; Allan Sheppard; Peter D Gluckman; Karen A Lillycrop; Graham C Burdge; Cameron McLean; Joanne Rodford; Joanne L Slater-Jefferies; Emma Garratt; Sarah R Crozier; B Starling Emerald; Catharine R Gale; Hazel M Inskip; Cyrus Cooper; Mark A Hanson Journal: Diabetes Date: 2011-04-06 Impact factor: 9.461
Authors: Lisa M Nicholas; Leewen Rattanatray; Severence M MacLaughlin; Susan E Ozanne; Dave O Kleemann; Simon K Walker; Janna L Morrison; Song Zhang; Beverley S Muhlhäusler; Malgorzata S Martin-Gronert; Isabella C McMillen Journal: FASEB J Date: 2013-05-31 Impact factor: 5.191