Catalina Alvarez1, Alejandra de-la-Torre2, Mónica Vargas3, Claudia Herrera4, Lored Dayana Uribe-Huertas3, Fabiana Lora3, Jorge Enrique Gómez-Marín3. 1. Grupo de Estudio en Parasitología Molecular, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia Grupo Toxoplasmosis y Parasitología General, CIMPAT, Universidad de los Andes. 2. Grupo de Estudio en Parasitología Molecular, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia Departamento de Ciencias Básicas, Universidad del Rosario, Bogotá, Colombia. 3. Grupo de Estudio en Parasitología Molecular, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia. 4. Grupo Toxoplasmosis y Parasitología General, CIMPAT, Universidad de los Andes Department of Tropical Medicine, Tulane University, School of Public Health and Tropical Medicine, New Orleans, Louisiana.
Abstract
BACKGROUND: ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in human toxoplasmosis. METHODS: We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. RESULTS: We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3%) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100%) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5%) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. CONCLUSIONS: The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains.
BACKGROUND: ROP16 is a protein kinase of Toxoplasma gondii identified in the mouse model as a virulent marker, but it is unknown whether this finding is relevant in humantoxoplasmosis. METHODS: We obtained the Toxoplasma ROP16 locus DNA sequence in samples from 12 patients with ocular toxoplasmosis, 1 sample from a patient with congenital toxoplasmosis, 22 samples from soldiers operating in the jungle, 2 samples from urban soldiers, and 10 samples from meat for human consumption. An enzyme-linked immunosorbent assay specific for antibodies against the ROP16 mouse-virulent peptide was performed in 46 serum specimens from patients with ocular toxoplasmosis and in 28 serum specimens from patients with chronic asymptomatic infection, of whom 19 had congenital infection and 11 had toxoplasmic lymphadenitis. RESULTS: We found a striking divergence of the ROP16 nucleotide sequences. Ten of 12 sequences (83.3%) from patients with ocular toxoplasmosis clustered with those of mouse-virulent strains, whereas 7 of 7 ROP16 sequences (100%) from meat were clustered with those of mouse-avirulent strains. Only 11 of 104 serum specimens (10.5%) had specific antibodies against the mouse-virulent peptide, and there was no association between clinical forms and positive results of serological assays. CONCLUSIONS: The majority of ROP16 nucleotide sequences from Colombian patients with ocular toxoplasmosis belonged to the group of mouse-virulent strains.
Authors: Jorge Enrique Gómez-Marín; Juliana Muñoz-Ortiz; Manuela Mejía-Oquendo; José Y Arteaga-Rivera; Nicolás Rivera-Valdivia; María Cristina Bohórquez-Granados; Stefany Velasco-Velásquez; Gabriela Castaño-de-la-Torre; John Alejandro Acosta-Dávila; Laura Lorena García-López; Elizabeth Torres-Morales; Mónica Vargas; Juan David Valencia; Daniel Celis-Giraldo; Alejandra de-la-Torre Journal: Heliyon Date: 2021-04-05
Authors: Mariangela Soberón Felín; Kanix Wang; Aliya Moreira; Andrew Grose; Karen Leahy; Ying Zhou; Fatima Alibana Clouser; Maryam Siddiqui; Nicole Leong; Perpetua Goodall; Morgan Michalowski; Mahmoud Ismail; Monica Christmas; Stephen Schrantz; Zuleima Caballero; Ximena Norero; Dora Estripeaut; David Ellis; Catalina Raggi; Catherine Castro; Davina Moossazadeh; Margarita Ramirez; Abhinav Pandey; Kevin Ashi; Samantha Dovgin; Ashtyn Dixon; Xuan Li; Ian Begeman; Sharon Heichman; Joseph Lykins; Delba Villalobos-Cerrud; Lorena Fabrega; José Luis Sanchez Montalvo; Connie Mendivil; Mario R Quijada; Silvia Fernández-Pirla; Valli de La Guardia; Digna Wong; Mayrene Ladrón de Guevara; Carlos Flores; Jovanna Borace; Anabel García; Natividad Caballero; Claudia Rengifo-Herrera; Maria Theresa Moreno de Saez; Michael Politis; Kristen Wroblewski; Theodore Karrison; Stephanie Ross; Mimansa Dogra; Vishan Dhamsania; Nicholas Graves; Marci Kirchberg; Kopal Mathur; Ashley Aue; Carlos M Restrepo; Alejandro Llanes; German Guzman; Arturo Rebellon; Kenneth Boyer; Peter Heydemann; A Gwendolyn Noble; Charles Swisher; Peter Rabiah; Shawn Withers; Teri Hull; Chunlei Su; Michael Blair; Paul Latkany; Ernest Mui; Daniel Vitor Vasconcelos-Santos; Alcibiades Villareal; Ambar Perez; Carlos Andrés Naranjo Galvis; Mónica Vargas Montes; Nestor Ivan Cardona Perez; Morgan Ramirez; Cy Chittenden; Edward Wang; Laura Lorena Garcia-López; Juliana Muñoz-Ortiz; Nicolás Rivera-Valdivia; María Cristina Bohorquez-Granados; Gabriela Castaño de-la-Torre; Guillermo Padrieu; Juan David Valencia Hernandez; Daniel Celis-Giraldo; Juan Alejandro Acosta Dávila; Elizabeth Torres; Manuela Mejia Oquendo; José Y Arteaga-Rivera; Dan L Nicolae; Andrey Rzhetsky; Nancy Roizen; Eileen Stillwaggon; Larry Sawers; Francois Peyron; Martine Wallon; Emanuelle Chapey; Pauline Levigne; Carmen Charter; Migdalia De Frias; Jose Montoya; Cindy Press; Raymund Ramirez; Despina Contopoulos-Ioannidis; Yvonne Maldonado; Oliver Liesenfeld; Carlos Gomez; Kelsey Wheeler; Ellen Holfels; David Frim; David McLone; Richard Penn; William Cohen; Samantha Zehar; James McAuley; Denis Limonne; Sandrine Houze; Sylvie Abraham; Raphael Piarroux; Vera Tesic; Kathleen Beavis; Ana Abeleda; Mari Sautter; Bouchra El Mansouri; Adlaoui El Bachir; Fatima Amarir; Kamal El Bissati; Alejandra de-la-Torre; Gabrielle Britton; Jorge Motta; Eduardo Ortega-Barria; Isabel Luz Romero; Paul Meier; Michael Grigg; Jorge Gómez-Marín; Jagannatha Rao Kosagisharaf; Xavier Sáez Llorens; Osvaldo Reyes; Rima McLeod Journal: Curr Pediatr Rep Date: 2022-08-22