Literature DB >> 25533614

Improved serotype-specific dengue virus detection in Trinidad and Tobago using a multiplex, real-time RT-PCR.

Jesse J Waggoner1, Nikita S D Sahadeo2, Arianne Brown3, Alisha Mohamed-Hadley4, Dexter Hadley4, Leslie Carrington3, Christine V F Carrington2, Benjamin A Pinsky5.   

Abstract

Dengue virus (DENV) transmission occurs throughout the Caribbean, though laboratory confirmation and epidemiologic surveillance are limited by the availability of serotype-specific molecular diagnostics. In this study, we show that a serotype-specific DENV multiplex, real-time reverse transcriptase-PCR (RT-PCR) detected DENV RNA in significantly more samples (82/182) than a reference hemi-nested RT-PCR (57/182; P=0.01).
Copyright © 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Dengue; Molecular; Pathology

Mesh：

Year:  2014        PMID： 25533614      PMCID： PMC4297500          DOI： 10.1016/j.diagmicrobio.2014.10.001

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  9 in total

1.  Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction.

Authors:  R S Lanciotti; C H Calisher; D J Gubler; G J Chang; A V Vorndam
Journal:  J Clin Microbiol       Date:  1992-03       Impact factor: 5.948

2.  Reply to "Inconclusive reverse transcription-PCR assay comparison for dengue virus detection and serotyping".

Authors:  Jesse J Waggoner; Benjamin A Pinsky
Journal:  J Clin Microbiol       Date:  2014-05       Impact factor: 5.948

3.  Inconclusive reverse transcription-PCR assay comparison for dengue virus detection and serotyping.

Authors:  Jorge L Muñoz-Jordán; Gilberto A Santiago
Journal:  J Clin Microbiol       Date:  2014-05       Impact factor: 5.948

4.  Invasion and maintenance of dengue virus type 2 and type 4 in the Americas.

Authors:  Christine V F Carrington; Jerome E Foster; Oliver G Pybus; Shannon N Bennett; Edward C Holmes
Journal:  J Virol       Date:  2005-12       Impact factor: 5.103

5.  Comparison of the FDA-approved CDC DENV-1-4 real-time reverse transcription-PCR with a laboratory-developed assay for dengue virus detection and serotyping.

Authors:  Jesse J Waggoner; Janaki Abeynayake; Malaya K Sahoo; Lionel Gresh; Yolanda Tellez; Karla Gonzalez; Gabriela Ballesteros; Frances P Guo; Angel Balmaseda; Kumudu Karunaratne; Eva Harris; Benjamin A Pinsky
Journal:  J Clin Microbiol       Date:  2013-07-31       Impact factor: 5.948

6.  Development of real-time reverse transcriptase PCR assays to detect and serotype dengue viruses.

Authors:  Li-Jung Chien; Tsai-Ling Liao; Pei-Yun Shu; Jyh-Hsiung Huang; Duane J Gubler; Gwong-Jen J Chang
Journal:  J Clin Microbiol       Date:  2006-04       Impact factor: 5.948

7.  Development of an internally controlled real-time reverse transcriptase PCR assay for pan-dengue virus detection and comparison of four molecular dengue virus detection assays.

Authors:  Jesse J Waggoner; Janaki Abeynayake; Malaya K Sahoo; Lionel Gresh; Yolanda Tellez; Karla Gonzalez; Gabriela Ballesteros; Angel Balmaseda; Kumudu Karunaratne; Eva Harris; Benjamin A Pinsky
Journal:  J Clin Microbiol       Date:  2013-05-01       Impact factor: 5.948

8.  Phylogeography and population dynamics of dengue viruses in the Americas.

Authors:  Orchid M Allicock; Philippe Lemey; Andrew J Tatem; Oliver G Pybus; Shannon N Bennett; Brandi A Mueller; Marc A Suchard; Jerome E Foster; Andrew Rambaut; Christine V F Carrington
Journal:  Mol Biol Evol       Date:  2012-01-06       Impact factor: 16.240

9.  Single-reaction, multiplex, real-time rt-PCR for the detection, quantitation, and serotyping of dengue viruses.

Authors:  Jesse J Waggoner; Janaki Abeynayake; Malaya K Sahoo; Lionel Gresh; Yolanda Tellez; Karla Gonzalez; Gabriela Ballesteros; Anna M Pierro; Paolo Gaibani; Frances P Guo; Vittorio Sambri; Angel Balmaseda; Kumudu Karunaratne; Eva Harris; Benjamin A Pinsky
Journal:  PLoS Negl Trop Dis       Date:  2013-04-18
  9 in total

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