| Literature DB >> 25529649 |
Dai-Hung Ngo1, Kyong-Hwa Kang1, BoMi Ryu2, Thanh-Sang Vo1, Won-Kyo Jung3, Hee-Guk Byun4, Se-Kwon Kim5.
Abstract
The aim of this study was to investigate antihypertensive effect of bioactive peptides from skate (Okamejei kenojei) skin gelatin. The Alcalase/protease gelatin hydrolysate below 1 kDa (SAP) exhibited the highest angiotensin-I converting enzyme (ACE) inhibition compared to other hydrolysates. SAP can decrease systolic blood pressure significantly in spontaneously hypertensive rats. SAP inhibited vasoconstriction via PPAR-γ expression, activation and phosphorylation of eNOS in lungs. Moreover, the expression levels of endothelin-1, RhoA, α-smooth muscle actin, cleaved caspase 3 and MAPK were decreased by SAP in lungs. Vascularity, muscularization and cellular proliferation in lungs were detected by immunohistochemical staining. Finally, two purified peptides (LGPLGHQ, 720Da and MVGSAPGVL, 829Da) showed potent ACE inhibition with IC50 values of 4.22 and 3.09 μM, respectively. These results indicate that bioactive peptides isolated from skate skin gelatin may serve as candidates against hypertension and could be used as functional food ingredients.Entities:
Keywords: Angiotensin converting enzyme inhibition; Antihypertension; PPAR-γ-eNOS; Skate peptides; Spontaneously hypertensive rats
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Year: 2014 PMID： 25529649 DOI： 10.1016/j.foodchem.2014.11.013
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514