W L Lü1, N Wang, P Gao, C Y Li, H S Zhao, Z T Zhang. 1. School of Stomatology, Capital Medical University, Beijing, 100050, China; Hospital and School of Stomatology, Tianjin Medical University, Tianjin, 300070, China.
Abstract
OBJECTIVES: To investigate effects of TiO2 nanotubes of different diameters on J744A.1 macrophage behaviour, secretion and expression of pro-inflammatory cytokines and chemokines. MATERIALS AND METHODS: Macrophage-like J744A.1 cells were cultured on three types of Ti surface: mechanically polished titanium plus 30 and 80 nm TiO2 nanotube surfaces, for 4, 24 and 48 h. Macrophage adhesion and proliferation were assessed using CCK-8 assay. Levels of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) and chemokines (MCP-1 and MIP-1α) secreted into the supernatant were measured using the Cytometric Bead Arrays kit. TNF-α, MCP-1 and MIP-1α gene expression were quantitatively analysed by real-time PCR. RESULTS: These show that TiO2 nanotube surfaces, especially of 80 nm TiO2 nanotube, benefited macrophage adhesion and proliferation, and reduced protein secretion and mRNA expression of TNF-α, MCP-1 and MIP-1α. IL-1β and IL-6 were undetectable on all the surfaces at all times. CONCLUSIONS: TiO2 nanotube surfaces, especially of 80 nm TiO2 nanotube, reduced inflammatory response in vitro, which might be part of a basis for rapid osseointegration in implants with TiO2 nanotube surfaces in animal studies.
OBJECTIVES: To investigate effects of TiO2 nanotubes of different diameters on J744A.1 macrophage behaviour, secretion and expression of pro-inflammatory cytokines and chemokines. MATERIALS AND METHODS: Macrophage-like J744A.1 cells were cultured on three types of Ti surface: mechanically polished titanium plus 30 and 80 nm TiO2 nanotube surfaces, for 4, 24 and 48 h. Macrophage adhesion and proliferation were assessed using CCK-8 assay. Levels of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) and chemokines (MCP-1 and MIP-1α) secreted into the supernatant were measured using the Cytometric Bead Arrays kit. TNF-α, MCP-1 and MIP-1α gene expression were quantitatively analysed by real-time PCR. RESULTS: These show that TiO2 nanotube surfaces, especially of 80 nm TiO2 nanotube, benefited macrophage adhesion and proliferation, and reduced protein secretion and mRNA expression of TNF-α, MCP-1 and MIP-1α. IL-1β and IL-6 were undetectable on all the surfaces at all times. CONCLUSIONS:TiO2 nanotube surfaces, especially of 80 nm TiO2 nanotube, reduced inflammatory response in vitro, which might be part of a basis for rapid osseointegration in implants with TiO2 nanotube surfaces in animal studies.
Authors: Seunghan Oh; Chiara Daraio; Li-Han Chen; Thomas R Pisanic; Rita R Fiñones; Sungho Jin Journal: J Biomed Mater Res A Date: 2006-07 Impact factor: 4.396
Authors: Edwin Lamers; X Frank Walboomers; Maciej Domanski; Ljupcho Prodanov; Jacoline Melis; Regina Luttge; Louis Winnubst; James M Anderson; Han J G E Gardeniers; John A Jansen Journal: Nanomedicine Date: 2011-06-24 Impact factor: 5.307
Authors: Bryan N Brown; Jolene E Valentin; Ann M Stewart-Akers; George P McCabe; Stephen F Badylak Journal: Biomaterials Date: 2009-01-01 Impact factor: 12.479
Authors: Clayton Farrugia; Alessandro Di Mauro; Frederick Lia; Edwin Zammit; Alex Rizzo; Vittorio Privitera; Giuliana Impellizzeri; Maria Antonietta Buccheri; Giancarlo Rappazzo; Maurice Grech; Paul Refalo; Stephen Abela Journal: Nanomaterials (Basel) Date: 2021-03-11 Impact factor: 5.076
Authors: Dimitrios Karazisis; Ahmed M Ballo; Sarunas Petronis; Hossein Agheli; Lena Emanuelsson; Peter Thomsen; Omar Omar Journal: Int J Nanomedicine Date: 2016-04-01