Literature DB >> 2549092

Enhanced infectivity of bluetongue virus in cell culture by centrifugation.

D R Sundin1, J O Mecham.   

Abstract

The effects of centrifugation of the infection of cell culture with bluetongue virus (BTV) were investigated. Baby hamster kidney cells were infected with BTV with or without centrifugation. Viral antigen was detected by immunofluorescence at 24 h in both centrifuged and noncentrifuged cultures. However, after 24 h of infection, the production of PFU in centrifuged cell cultures was 10- to 20-fold greater than that seen in cultures not centrifuged. In addition, centrifugation enhanced the direct detection of PFU from blood samples collected from a sheep experimentally infected with BTV.

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Year:  1989        PMID: 2549092      PMCID: PMC267635          DOI: 10.1128/jcm.27.7.1659-1660.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  9 in total

1.  Rapid detection of cytomegalovirus pulmonary infection by bronchoalveolar lavage and centrifugation culture.

Authors:  S W Crawford; R A Bowden; R C Hackman; C A Gleaves; J D Meyers; J G Clark
Journal:  Ann Intern Med       Date:  1988-02       Impact factor: 25.391

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Authors:  J B Hudson; V Misra; T R Mosmann
Journal:  Virology       Date:  1976-07-01       Impact factor: 3.616

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Authors:  M M Jochim
Journal:  Prog Clin Biol Res       Date:  1985

Review 4.  WHO/FAO Working Team Report: virology.

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Journal:  Prog Clin Biol Res       Date:  1985

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Authors:  W J Martin; T F Smith
Journal:  J Clin Microbiol       Date:  1986-06       Impact factor: 5.948

6.  Characterization of bluetongue virus ribonucleic acid.

Authors:  D W Verwoerd; H Louw; R A Oellermann
Journal:  J Virol       Date:  1970-01       Impact factor: 5.103

7.  Structure of the bluetongue virus capsid.

Authors:  D W Verwoerd; H J Els; E M De Villiers; H Huismans
Journal:  J Virol       Date:  1972-10       Impact factor: 5.103

8.  Further studies on the mechanism of centrifugal enhancement of cytomegalovirus infectivity.

Authors:  J B Hudson
Journal:  J Virol Methods       Date:  1988-02       Impact factor: 2.014

9.  Detection of herpes simplex virus in conventional tube cell cultures and in shell vials with a DNA probe kit and monoclonal antibodies.

Authors:  M J Espy; T F Smith
Journal:  J Clin Microbiol       Date:  1988-01       Impact factor: 5.948

  9 in total
  7 in total

Review 1.  Physical and chemical methods for enhancing rapid detection of viruses and other agents.

Authors:  J H Hughes
Journal:  Clin Microbiol Rev       Date:  1993-04       Impact factor: 26.132

2.  Spinoculation triggers dynamic actin and cofilin activity that facilitates HIV-1 infection of transformed and resting CD4 T cells.

Authors:  Jia Guo; Weifeng Wang; Dongyang Yu; Yuntao Wu
Journal:  J Virol       Date:  2011-07-27       Impact factor: 5.103

3.  Centrifugal enhancement of hepatitis C virus infection of human hepatocytes.

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Journal:  J Virol Methods       Date:  2008-03       Impact factor: 2.014

4.  An updated review on bluetongue virus: epidemiology, pathobiology, and advances in diagnosis and control with special reference to India.

Authors:  Mani Saminathan; Karam Pal Singh; Jaynudin Hajibhai Khorajiya; Murali Dinesh; Sobharani Vineetha; Madhulina Maity; At Faslu Rahman; Jyoti Misri; Yashpal Singh Malik; Vivek Kumar Gupta; Raj Kumar Singh; Kuldeep Dhama
Journal:  Vet Q       Date:  2020-12       Impact factor: 3.320

5.  Oncolytic bluetongue viruses: promise, progress, and perspectives.

Authors:  Joseph K-K Li
Journal:  Front Microbiol       Date:  2011-03-16       Impact factor: 5.640

6.  Spinoculation Enhances HBV Infection in NTCP-Reconstituted Hepatocytes.

Authors:  Ran Yan; Yongmei Zhang; Dawei Cai; Yuanjie Liu; Andrea Cuconati; Haitao Guo
Journal:  PLoS One       Date:  2015-06-12       Impact factor: 3.240

7.  Enhancement of feline infectious peritonitis virus type I infection in cell cultures using low-speed centrifugation.

Authors:  T Hohdatsu; T Tatekawa; H Koyama
Journal:  J Virol Methods       Date:  1995-02       Impact factor: 2.014

  7 in total

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