| Literature DB >> 25487215 |
Abstract
High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNA-poly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specific RNA-Seq libraries from varying quantities of total RNA.Mesh：
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Year: 2015 PMID： 25487215 DOI： 10.1007/978-1-4939-2175-1_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745