UNLABELLED: The objective of this study was to examine whether 7 SNPs previously associated with obesity-related traits that add or remove potential sites of DNA methylation are accompanied by differential DNA methylation and subsequently affect adiposity variables or body weight reduction in WBC from obese subjects under an energy-restricted program. MATERIAL AND METHODS: Anthropometric measurements were assessed in 47 volunteers recruited within the RESMENA study (Spain). At baseline, DNA from white blood cells was isolated and 7 obesity-related trait CpG-SNPs were genotyped by TaqMan-PCR. Then, methylation levels of CpG-SNP sites were quantified by MassArray® EpiTyper™ or MS-HRM approaches. RESULTS: Differential DNA methylation levels were observed by genotypes in all of the CpG-SNPs analyzed. The FTO and BDNF methylation levels were further correlated with baseline body weight and, BDNF mRNA levels and body weight change, respectively. Moreover, the rs7359397 (SH2B1) was associated with the body weight, body mass index, and truncal fat mass reduction. CONCLUSIONS: Our results reveal the interaction of epigenetic and genetic variations in CpG-SNPs, especially in BDNF and SH2B1 genes, and how allele-specific methylation may contribute to elucidate the possible molecular mechanisms as these SNPs are affecting the decrease of mRNA levels and contributing to a lower body weight reduction.
UNLABELLED: The objective of this study was to examine whether 7 SNPs previously associated with obesity-related traits that add or remove potential sites of DNA methylation are accompanied by differential DNA methylation and subsequently affect adiposity variables or body weight reduction in WBC from obese subjects under an energy-restricted program. MATERIAL AND METHODS: Anthropometric measurements were assessed in 47 volunteers recruited within the RESMENA study (Spain). At baseline, DNA from white blood cells was isolated and 7 obesity-related trait CpG-SNPs were genotyped by TaqMan-PCR. Then, methylation levels of CpG-SNP sites were quantified by MassArray® EpiTyper™ or MS-HRM approaches. RESULTS: Differential DNA methylation levels were observed by genotypes in all of the CpG-SNPs analyzed. The FTO and BDNF methylation levels were further correlated with baseline body weight and, BDNF mRNA levels and body weight change, respectively. Moreover, the rs7359397 (SH2B1) was associated with the body weight, body mass index, and truncal fat mass reduction. CONCLUSIONS: Our results reveal the interaction of epigenetic and genetic variations in CpG-SNPs, especially in BDNF and SH2B1 genes, and how allele-specific methylation may contribute to elucidate the possible molecular mechanisms as these SNPs are affecting the decrease of mRNA levels and contributing to a lower body weight reduction.
Authors: Francesca Pirini; Sebastian Rodriguez-Torres; Bola Grace Ayandibu; María Orera-Clemente; Alberto Gonzalez-de la Vega; Fahcina Lawson; Roland J Thorpe; David Sidransky; Rafael Guerrero-Preston Journal: Mol Med Rep Date: 2017-11-14 Impact factor: 2.952
Authors: Kerstin Rohde; Matthias Klös; Lydia Hopp; Xuanshi Liu; Maria Keller; Michael Stumvoll; Arne Dietrich; Michael R Schön; Daniel Gärtner; Tobias Lohmann; Miriam Dreßler; Peter Kovacs; Hans Binder; Matthias Blüher; Yvonne Böttcher Journal: Sci Rep Date: 2017-09-28 Impact factor: 4.379
Authors: Sarah Voisin; Markus Sällman Almén; Galina Y Zheleznyakova; Lina Lundberg; Sanaz Zarei; Sandra Castillo; Fia Ence Eriksson; Emil K Nilsson; Matthias Blüher; Yvonne Böttcher; Peter Kovacs; Janis Klovins; Mathias Rask-Andersen; Helgi B Schiöth Journal: Genome Med Date: 2015-10-08 Impact factor: 11.117