| Literature DB >> 25470546 |
Bas Brouwers1, Geoffroy de Faudeur2, Anna B Osipovich3, Lotte Goyvaerts2, Katleen Lemaire2, Leen Boesmans2, Elisa J G Cauwelier1, Mikaela Granvik2, Vincent P E G Pruniau1, Leentje Van Lommel2, Jolien Van Schoors4, Jennifer S Stancill3, Ilse Smolders4, Vincent Goffin5, Nadine Binart6, Peter in't Veld7, Jeroen Declercq1, Mark A Magnuson3, John W M Creemers8, Frans Schuit9, Anica Schraenen2.
Abstract
The human growth hormone (hGH) minigene is frequently used in the derivation of transgenic mouse lines to enhance transgene expression. Although this minigene is present in the transgenes as a secondcistron, and thus not thought to be expressed, we found that three commonly used lines, Pdx1-Cre(Late), RIP-Cre, and MIP-GFP, each expressed significant amounts of hGH in pancreatic islets. Locally secreted hGH binds to prolactin receptors on β cells, activates STAT5 signaling, and induces pregnancy-like changes in gene expression, thereby augmenting pancreatic β cell mass and insulin content. In addition, islets of Pdx1-Cre(Late) mice have lower GLUT2 expression and reduced glucose-induced insulin release and are protected against the β cell toxin streptozotocin. These findings may be important when interpreting results obtained when these and other hGH minigene-containing transgenic mice are used.Entities:
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Year: 2014 PMID： 25470546 PMCID： PMC5674787 DOI： 10.1016/j.cmet.2014.11.004
Source DB: PubMed Journal: Cell Metab ISSN： 1550-4131 Impact factor: 27.287