AIM: To investigate if azathioprine could reduce adenoma formation in Apc(Min/+) , a mouse model of sporadic intestinal tumorigenesis. METHODS: Azathioprine was administered via drinking water (estimated 6-20 mg/kg body weight per day) to Apc(Min/+) and wildtype mice. Control animals received vehicle only (DMSO) dissolved in drinking water. At 15 wk of age all mice were sacrificed and intestines of Apc(Min/+) were harvested for evaluation of polyp number. Azathioprine induced toxicity was investigated by immunohistochemical analysis on spleens. RESULTS: All azathioprine treated mice showed signs of drug-associated toxicity such as weight loss and development of splenic T-cell lymphomas. Although this suggests that the thiopurine concentration was clearly in the therapeutic range, it did not reduce tumor formation (48 ± 3.1 adenomas vs 59 ± 5.7 adenomas, P = 0.148). CONCLUSION: We conclude that in the absence of inflammation, azathioprine does not affect intestinal tumorigenesis.
AIM: To investigate if azathioprine could reduce adenoma formation in Apc(Min/+) , a mouse model of sporadic intestinal tumorigenesis. METHODS:Azathioprine was administered via drinking water (estimated 6-20 mg/kg body weight per day) to Apc(Min/+) and wildtype mice. Control animals received vehicle only (DMSO) dissolved in drinking water. At 15 wk of age all mice were sacrificed and intestines of Apc(Min/+) were harvested for evaluation of polyp number. Azathioprine induced toxicity was investigated by immunohistochemical analysis on spleens. RESULTS: All azathioprine treated mice showed signs of drug-associated toxicity such as weight loss and development of splenic T-cell lymphomas. Although this suggests that the thiopurine concentration was clearly in the therapeutic range, it did not reduce tumor formation (48 ± 3.1 adenomas vs 59 ± 5.7 adenomas, P = 0.148). CONCLUSION: We conclude that in the absence of inflammation, azathioprine does not affect intestinal tumorigenesis.
Authors: Jarom Heijmans; Mattheus C B Wielenga; Sanne Liesbeth Rosekrans; Jooske F van Lidth de Jeude; Joris Roelofs; Patrick Groothuis; Antwan Ederveen; Eveline S M de Jonge-Muller; Izak Biemond; James C H Hardwick; Geert D'Haens; Daniel W Hommes; Vanesa Muncan; Gijs R van den Brink Journal: Gut Date: 2013-02-13 Impact factor: 23.059
Authors: Nabeel Khan; Ali M Abbas; Gary R Lichtenstein; Edward V Loftus; Lydia A Bazzano Journal: Gastroenterology Date: 2013-07-25 Impact factor: 22.682
Authors: Ana Teresa P Carvalho; Barbara C Esberard; Renata S B Fróes; Davy C M Rapozo; Ana B Grinman; Tatiana A Simão; Juliana C V C Santos; Antonio José V Carneiro; Luis Felipe Ribeiro-Pinto; Heitor S P de Souza Journal: World J Gastroenterol Date: 2014-03-28 Impact factor: 5.742
Authors: Liene Elsone; Joanna Kitley; Sebastian Luppe; Daniel Lythgoe; Kerry Mutch; Saiju Jacob; Rachel Brown; Kathryn Moss; Benjamin McNeillis; Yee Yen Goh; M Isabel Leite; Neil Robertson; Jackie Palace; Anu Jacob Journal: Mult Scler Date: 2014-03-19 Impact factor: 6.312