INTRODUCTION: The need to control dengue transmission by means of insecticides has led to the development of resistance to most of the products used worldwide against mosquitoes. In the State of São Paulo, the Superintendência de Controle de Endemias (SUCEN) has annually monitored the susceptibility of Aedes aegypti to insecticides since 1996; since 1999, surveys were conducted in collaboration with the National Network of Laboratories (MoReNAa Network) and were coordinated by the Ministry of Health. In this study, in addition to the biological characterization of insecticide resistance in the laboratory, the impact of resistance on field control was evaluated for vector populations that showed resistance in laboratory assays. METHODS: Field efficacy tests with larvicides and adulticides were performed over a 13-year period, using World Health Organization protocols. RESULTS: Data from the field tests showed a reduction in the residual effect of temephos on populations with a resistance ratio of 3. For adults, field control was less effective in populations characterized as resistant in laboratory qualitative assays, and this was confirmed using qualitative assays and field evaluation. CONCLUSIONS: Our results indicated that management of resistance development needs to be adopted when insect populations show reduced susceptibility. The use of insecticides is a self-limiting tool that needs to be applied cautiously, and dengue control requires more sustainable strategies.
INTRODUCTION: The need to control dengue transmission by means of insecticides has led to the development of resistance to most of the products used worldwide against mosquitoes. In the State of São Paulo, the Superintendência de Controle de Endemias (SUCEN) has annually monitored the susceptibility of Aedes aegypti to insecticides since 1996; since 1999, surveys were conducted in collaboration with the National Network of Laboratories (MoReNAa Network) and were coordinated by the Ministry of Health. In this study, in addition to the biological characterization of insecticide resistance in the laboratory, the impact of resistance on field control was evaluated for vector populations that showed resistance in laboratory assays. METHODS: Field efficacy tests with larvicides and adulticides were performed over a 13-year period, using World Health Organization protocols. RESULTS: Data from the field tests showed a reduction in the residual effect of temephos on populations with a resistance ratio of 3. For adults, field control was less effective in populations characterized as resistant in laboratory qualitative assays, and this was confirmed using qualitative assays and field evaluation. CONCLUSIONS: Our results indicated that management of resistance development needs to be adopted when insect populations show reduced susceptibility. The use of insecticides is a self-limiting tool that needs to be applied cautiously, and dengue control requires more sustainable strategies.
Authors: Luiz Paulo Brito; Luana Carrara; Rafael Maciel de Freitas; José Bento Pereira Lima; Ademir J Martins Journal: Biomed Res Int Date: 2018-07-11 Impact factor: 3.411
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Authors: Mateus Chediak; Fabiano G Pimenta; Giovanini E Coelho; Ima A Braga; José Bento P Lima; Karina Ribeiro Lj Cavalcante; Lindemberg C de Sousa; Maria Alice V de Melo-Santos; Maria de Lourdes da G Macoris; Ana Paula de Araújo; Constância Flávia J Ayres; Maria Teresa M Andrighetti; Ricristhi Gonçalves de A Gomes; Kauara B Campos; Raul Narciso C Guedes Journal: Mem Inst Oswaldo Cruz Date: 2016-04-29 Impact factor: 2.743
Authors: Maria de Lourdes Macoris; Ademir Jesus Martins; Maria Teresa Macoris Andrighetti; José Bento Pereira Lima; Denise Valle Journal: PLoS Negl Trop Dis Date: 2018-03-30