Literature DB >> 25448393

Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru.

C G Duarte1, C Bonilla2, G Guimarães1, R A Machado de Avila3, T M Mendes1, W Silva2, B Tintaya2, A Yarleque4, C Chávez-Olórtegui5.   

Abstract

In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.
Copyright © 2014 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Antivenom; Immunization; Loxosceles; Neutralization; Recombinant protein

Mesh：

Substances：

Year:  2014        PMID： 25448393     DOI： 10.1016/j.toxicon.2014.10.023

Source DB:  PubMed          Journal:  Toxicon        ISSN： 0041-0101            Impact factor:   3.033


  9 in total

1.  Clinical Effects of the Immunization Protocol Using Loxosceles Venom in Naïve Horses.

Authors:  Ana Luísa Soares de Miranda; Bruno Cesar Antunes; João Carlos Minozzo; Sabrina de Almeida Lima; Ana Flávia Machado Botelho; Marco Túlio Gomes Campos; Carlos Delfin Chávez-Olórtegui; Benito Soto-Blanco
Journal:  Toxins (Basel)       Date:  2022-05-13       Impact factor: 5.075

Review 2.  Biotechnological potential of Phospholipase D for Loxosceles antivenom development.

Authors:  Matías Fingermann; Adolfo Rafael de Roodt; Osvaldo Cascone; María Victoria Miranda
Journal:  Toxicon X       Date:  2020-04-18

Review 3.  Innovative Immunization Strategies for Antivenom Development.

Authors:  Erick Bermúdez-Méndez; Albert Fuglsang-Madsen; Sofie Føns; Bruno Lomonte; José María Gutiérrez; Andreas Hougaard Laustsen
Journal:  Toxins (Basel)       Date:  2018-11-02       Impact factor: 4.546

4.  Design and Production of a Recombinant Hybrid Toxin to Raise Protective Antibodies Against Loxosceles Spider Venom.

Authors:  Paula A L Calabria; Lhiri Hanna A L Shimokava-Falcao; Monica Colombini; Ana M Moura-da-Silva; Katia C Barbaro; Eliana L Faquim-Mauro; Geraldo S Magalhaes
Journal:  Toxins (Basel)       Date:  2019-02-12       Impact factor: 4.546

Review 5.  Brown Spider (Loxosceles) Venom Toxins as Potential Biotools for the Development of Novel Therapeutics.

Authors:  Daniele Chaves-Moreira; Fernando Hitomi Matsubara; Zelinda Schemczssen-Graeff; Elidiana De Bona; Vanessa Ribeiro Heidemann; Clara Guerra-Duarte; Luiza Helena Gremski; Carlos Chávez-Olórtegui; Andrea Senff-Ribeiro; Olga Meiri Chaim; Raghuvir Krishnaswamy Arni; Silvio Sanches Veiga
Journal:  Toxins (Basel)       Date:  2019-06-19       Impact factor: 4.546

6.  Loxoscelism: Advances and Challenges in the Design of Antibody Fragments with Therapeutic Potential.

Authors:  Sabrina Karim-Silva; Alessandra Becker-Finco; Isabella Gizzi Jiacomini; Fanny Boursin; Arnaud Leroy; Magali Noiray; Juliana de Moura; Nicolas Aubrey; Philippe Billiald; Larissa M Alvarenga
Journal:  Toxins (Basel)       Date:  2020-04-16       Impact factor: 4.546

7.  A Web of Coagulotoxicity: Failure of Antivenom to Neutralize the Destructive (Non-Clotting) Fibrinogenolytic Activity of Loxosceles and Sicarius Spider Venoms.

Authors:  Dwin Grashof; Christina N Zdenek; James S Dobson; Nicholas J Youngman; Francisco Coimbra; Melisa Benard-Valle; Alejandro Alagon; Bryan G Fry
Journal:  Toxins (Basel)       Date:  2020-01-30       Impact factor: 4.546

8.  TCTP from Loxosceles Intermedia (Brown Spider) Venom Contributes to the Allergic and Inflammatory Response of Cutaneous Loxoscelism.

Authors:  Marianna Boia-Ferreira; Kamila G Moreno; Alana B C Basílio; Lucas P da Silva; Larissa Vuitika; Bruna Soley; Ana Carolina M Wille; Lucélia Donatti; Katia C Barbaro; Olga M Chaim; Luiza Helena Gremski; Silvio S Veiga; Andrea Senff-Ribeiro
Journal:  Cells       Date:  2019-11-22       Impact factor: 6.600

Review 9.  Prospective Use of Brown Spider Venom Toxins as Therapeutic and Biotechnological Inputs.

Authors:  Luiza Helena Gremski; Fernando Hitomi Matsubara; Nayanne Louise Costacurta Polli; Bruno Cesar Antunes; Pedro Henrique de Caires Schluga; Hanna Câmara da Justa; João Carlos Minozzo; Ana Carolina Martins Wille; Andrea Senff-Ribeiro; Silvio Sanches Veiga
Journal:  Front Mol Biosci       Date:  2021-06-17
  9 in total

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