Wu Ren-Pei1, Xi Hui-Jun1, Qi Ke1, Wang Dong1, Nie Xing2, Li Zhao-Shen3. 1. Department of Gastroenterology, Changhai Hospital of Second Military Medical University, Shanghai, China. 2. National Center for Nanoscience and Technology of Chinese Academy of Sciences, Beijing, China. 3. Department of Gastroenterology, Changhai Hospital of Second Military Medical University, Shanghai, China. Electronic address: dr_lizhaoshen@126.com.
Abstract
BACKGROUND: The purpose of this article was to investigate bacterial biofilm formed on endoscopes and to explore the possible correlation between endoscope reprocessing procedures and bacterial biofilm growth on endoscope channels. METHODS: Sixty-six endoscope suction and biopsy channels and 13 water and air channels were collected from 66 hospitals throughout China. Scanning electron microscopy was used to observe biofilm growth on the internal surface of these channels. Questionnaires were mailed to 66 endoscopy centers to investigate reprocessing procedures for endoscopes. RESULTS: Obvious biofilm growth was detected on 36 suction and biopsy channels (36/66, 54.6%) and 10 water and air channels (10/13, 76.9%). The percentage of manual cleaning in group B (n = 36, without detection of biofilms) was 92.3% (33/36), whereas it was 50.0% (15/30) in group A (n = 30, with detection of biofilms). Follow-up of group A (n = 30) showed that no biofilm was detected, whereas biofilm was detected in group B. The difference was statistically significant (P = .001). The proportion of detergent reuse in group B was 92.3% (33/36), and it was 61.5% in group A (18/30) (P = .005). The proportion of alcohol-air drying in group B was 38.9% (14/36), and it was 76.7% (23/30) in group A (P = .002). CONCLUSION: The formation of endoscopic biofilm during clinical practice may be related to reuse of detergent, manual cleaning, and incomplete drying.
BACKGROUND: The purpose of this article was to investigate bacterial biofilm formed on endoscopes and to explore the possible correlation between endoscope reprocessing procedures and bacterial biofilm growth on endoscope channels. METHODS: Sixty-six endoscope suction and biopsy channels and 13 water and air channels were collected from 66 hospitals throughout China. Scanning electron microscopy was used to observe biofilm growth on the internal surface of these channels. Questionnaires were mailed to 66 endoscopy centers to investigate reprocessing procedures for endoscopes. RESULTS: Obvious biofilm growth was detected on 36 suction and biopsy channels (36/66, 54.6%) and 10 water and air channels (10/13, 76.9%). The percentage of manual cleaning in group B (n = 36, without detection of biofilms) was 92.3% (33/36), whereas it was 50.0% (15/30) in group A (n = 30, with detection of biofilms). Follow-up of group A (n = 30) showed that no biofilm was detected, whereas biofilm was detected in group B. The difference was statistically significant (P = .001). The proportion of detergent reuse in group B was 92.3% (33/36), and it was 61.5% in group A (18/30) (P = .005). The proportion of alcohol-air drying in group B was 38.9% (14/36), and it was 76.7% (23/30) in group A (P = .002). CONCLUSION: The formation of endoscopic biofilm during clinical practice may be related to reuse of detergent, manual cleaning, and incomplete drying.
Authors: Gheorghe G Balan; Catalin Victor Sfarti; Stefan Andrei Chiriac; Carol Stanciu; Anca Trifan Journal: Eur J Clin Microbiol Infect Dis Date: 2019-09-03 Impact factor: 3.267
Authors: Michelle J Alfa; Harminder Singh; Zoann Nugent; Donald Duerksen; Gale Schultz; Carol Reidy; Patricia DeGagne; Nancy Olson Journal: Front Med (Lausanne) Date: 2017-11-07
Authors: Charles Eugenio McCafferty; Marra Jai Aghajani; David Abi-Hanna; Iain Bruce Gosbell; Slade Owen Jensen Journal: Ann Clin Microbiol Antimicrob Date: 2018-10-10 Impact factor: 3.944
Authors: Maria Letícia de Miranda Mati; Natália Rocha Guimarães; Paula Prazeres Magalhães; Luiz de Macêdo Farias; Adriana Cristina de Oliveira Journal: Rev Lat Am Enfermagem Date: 2019-12-05