H Xu1, H W Jiang2, Q Ding1. 1. Departamento de Urología, Hospital Huashan, Universidad Fudan, Shanghai, PR China. 2. Departamento de Urología, Hospital Huashan, Universidad Fudan, Shanghai, PR China. Electronic address: haowen_jiang@126.com.
Abstract
INTRODUCTION: We aimed to investigate the role of IGF-1 related pathway in high-fat diet (HFD) promotion of TRAMP mouse PCa progression. METHODS: TRAMP mice were randomly divided into two groups: HFD group and normal diet group. TRAMP mice of both groups were sacrificed and sampled on the 20th, 24th and 28th week respectively. Serum levels of insulin, IGF-1 and IGF-2 were tested by ELISA. Prostate tissue of TRAMP mice was used for both HE staining and immunohistochemical staining of IGF-1 related pathway proteins, including IGF-1Rα, IGF -1Rβ, IGFBPs and AKT. RESULTS: The mortality of TRAMP mice from HFD group was significantly higher than that of normal diet group (23.81% and 7.14%, p=.035). The tumor incidence of HFD TRAMP mice at 20(th) week was significantly higher than normal diet group (78.57% and 35.71%, p=.022). Serum IGF-1 level of HFD TRAMP mice was significantly higher than that of normal diet TRAMP mice. Serum IGF-1 level tended to increase with HFD TRAMP mice's age. HFD TRAMP mice had higher positive staining rate of IGF-1Rα, IGF-1Rβ, IGFBP3 and Akt than normal diet TRAMP mice. CONCLUSIONS: IGF-1 related pathway played an important role in high-fat diet promotion of TRAMP mouse PCa development and progression.
INTRODUCTION: We aimed to investigate the role of IGF-1 related pathway in high-fat diet (HFD) promotion of TRAMP mouse PCa progression. METHODS: TRAMP mice were randomly divided into two groups: HFD group and normal diet group. TRAMP mice of both groups were sacrificed and sampled on the 20th, 24th and 28th week respectively. Serum levels of insulin, IGF-1 and IGF-2 were tested by ELISA. Prostate tissue of TRAMP mice was used for both HE staining and immunohistochemical staining of IGF-1 related pathway proteins, including IGF-1Rα, IGF -1Rβ, IGFBPs and AKT. RESULTS: The mortality of TRAMP mice from HFD group was significantly higher than that of normal diet group (23.81% and 7.14%, p=.035). The tumor incidence of HFD TRAMP mice at 20(th) week was significantly higher than normal diet group (78.57% and 35.71%, p=.022). Serum IGF-1 level of HFD TRAMP mice was significantly higher than that of normal diet TRAMP mice. Serum IGF-1 level tended to increase with HFD TRAMP mice's age. HFD TRAMP mice had higher positive staining rate of IGF-1Rα, IGF-1Rβ, IGFBP3 and Akt than normal diet TRAMP mice. CONCLUSIONS:IGF-1 related pathway played an important role in high-fat diet promotion of TRAMP mouse PCa development and progression.
Keywords:
Cáncer de próstata; Dieta alta en grasa; Factor de crecimiento tipo insulina; High-fat diet; Insulin like growth factor; Prostate cancer; TRAMP
Authors: Justin R Gregg; Jiali Zheng; David S Lopez; Chad Reichard; Gladys Browman; Brian Chapin; Jeri Kim; John Davis; Carrie R Daniel Journal: Br J Cancer Date: 2019-01-25 Impact factor: 7.640