Optimization of enzymolysis-ultrasonic assisted extraction of polysaccharides from Momordica charabtia L. by response surface methodology.

An efficient enzymolysis-ultrasonic assisted extraction (EUAE) was developed and optimized for the extraction of polysaccharide from Momordica charabtia L. The single factor experiments and orthogonal experiments were used for the key experimental factors and their test range. Based on the preliminary experimental results, the response surface methodology (RSM) and Box-Behnken design (BBD) were applied for the optimization of EUAE conditions. Using the multiple regression analysis and analysis of variance (ANOVA), the experimental data were fitted to a second-order polynomial equation and were used to generate the mathematical model of optimization experiments. The optimal extraction conditions were as follows: a pH of 4.38, a extraction temperature of 52.02°C and a extraction time of 36.87 min. Under the optimal extraction conditions, the extraction yield of Momordica charabtia L. polysaccharides (MCP) was 29.75±0.48%, which was well matched with the predicted value (29.80%) of the BBD model.