Literature DB >> 25433463

Evaluation of zraP gene expression characteristics and construction of a lead (Pb) sensing and removal system in a recombinant Escherichia coli.

Murali Kannan Maruthamuthu1, Irisappan Ganesh, Sambandam Ravikumar, Soon Ho Hong.   

Abstract

A ZraP-based lead sensing and removal system was constructed in E. coli. It was regulated by the ZraS/ZraR two-component system. The expression profile of the zraP gene towards extracellular lead was studied via real-time PCR. A dual-function bacterial system was also designed to express GFP and OmpC-lead binding peptide under the control of zraP for the simultaneous sensing and adsorption of environmental lead without additional manipulation. The constructed bacterial system can emit fluorescence and it adsorbed a maximum of 487 µmol lead/g cell DCW. From a study of artificial wastewater, the constructed bacteria adsorbed lead highly selectively (427 µmol lead/g cell DCW) among other metal ions. The newly-constructed dual function bacterial system can be applied for the development of an efficient process for the removal of lead from polluted wastes.

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Year:  2014        PMID： 25433463     DOI： 10.1007/s10529-014-1732-x

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  10 in total

1.  Manganese and cobalt recovery by surface display of metal binding peptide on various loops of OmpC in Escherichia coli.

Authors:  Murali Kannan Maruthamuthu; Vidhya Selvamani; Saravanan Prabhu Nadarajan; Hyungdon Yun; You-Kwan Oh; Gyeong Tae Eom; Soon Ho Hong
Journal:  J Ind Microbiol Biotechnol       Date:  2017-11-28       Impact factor: 3.346

2.  In vivo biosensors: mechanisms, development, and applications.

Authors:  Shuobo Shi; Ee Lui Ang; Huimin Zhao
Journal:  J Ind Microbiol Biotechnol       Date:  2018-01-29       Impact factor: 3.346

Review 3.  Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery.

Authors:  Sambandam Ravikumar; Mary Grace Baylon; Si Jae Park; Jong-Il Choi
Journal:  Microb Cell Fact       Date:  2017-04-14       Impact factor: 5.328

4.  Recombinant Fusion Protein PbrD Cross-Linked to Calcium Alginate Nanoparticles for Pb Remediation.

Authors:  Vidya Keshav; Paul Franklyn; Kulsum Kondiah
Journal:  ACS Omega       Date:  2019-10-04

5.  Development of Cadmium Multiple-Signal Biosensing and Bioadsorption Systems Based on Artificial Cad Operons.

Authors:  Yan Guo; Chang-Ye Hui; Nai-Xing Zhang; Lisa Liu; Hui Li; Hong-Ju Zheng
Journal:  Front Bioeng Biotechnol       Date:  2021-02-10

6.  Molecular Studies on the Nephroprotective Potential of Celastrus paniculatus against Lead-Acetate-Induced Nephrotoxicity in Experimental Rats: Role of the PI3K/AKT Signaling Pathway.

Authors:  Karunakaran Balaji; Jagadish Vijayakumar; Ponnusamy Kasirajan Sankaran; Sivanesan Senthilkumar; Rajagopalan Vijayaraghavan; Jayaraman Selvaraj; Maria Francis Yuvaraj
Journal:  Molecules       Date:  2021-11-02       Impact factor: 4.411

7.  Thyroid-Stimulating Hormone Favors Runx2-Mediated Matrix Mineralization in HOS and SaOS2 Cells: An In Vitro and In Silico Approach.

Authors:  Ramajayam Govindan; Mohamed El-Sherbiny; Khalid Mohamed Morsy Ibraheem; Srinivasan Narasimhan; Mohamed El-Dosoky Mohamed Salama; Fazil Ahmad; Selvaraj Jayaraman; Vishnu Priya Veeraraghavan; Srinivasan Vengadassalapathy; Surapaneni Krishna Mohan; Vidhya Rekha Umapathy; Gayathri Rengasamy; Shazia Fathima Jaffer Hussain; Maheshkumar Poomarimuthu; Senthilkumar Kalimuthu
Journal:  Molecules       Date:  2022-01-18       Impact factor: 4.411

Review 8.  A Comprehensive Review on Therapeutic Perspectives of Phytosterols in Insulin Resistance: A Mechanistic Approach.

Authors:  Monisha Prasad; Selvaraj Jayaraman; Mohamed Ahmed Eladl; Mohamed El-Sherbiny; Mosaab Abdella Ebrahim Abdelrahman; Vishnu Priya Veeraraghavan; Srinivasan Vengadassalapathy; Vidhya Rekha Umapathy; Shazia Fathima Jaffer Hussain; Kalaiselvi Krishnamoorthy; Durairaj Sekar; Chella Perumal Palanisamy; Surapaneni Krishna Mohan; Ponnulakshmi Rajagopal
Journal:  Molecules       Date:  2022-02-28       Impact factor: 4.411

9.  Pull-Down Into Active Inclusion Bodies and Their Application in the Detection of (Poly)-Phosphates and Metal-Ions.

Authors:  Eva Hrabarova; Martina Belkova; Romana Koszagova; Jozef Nahalka
Journal:  Front Bioeng Biotechnol       Date:  2022-03-01

10.  Stevioside Attenuates Insulin Resistance in Skeletal Muscle by Facilitating IR/IRS-1/Akt/GLUT 4 Signaling Pathways: An In Vivo and In Silico Approach.

Authors:  Abilasha Deenadayalan; Vijayalakshmi Subramanian; Vijayalakshmi Paramasivan; Vishnu Priya Veeraraghavan; Gayathri Rengasamy; Janaki Coiambatore Sadagopan; Ponnulakshmi Rajagopal; Selvaraj Jayaraman
Journal:  Molecules       Date:  2021-12-20       Impact factor: 4.411
  10 in total

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