Literature DB >> 25431075

Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA).

Antón Barreiro-Iglesias1, Michael I Shifman.   

Abstract

Here, we present a detailed protocol for the detection of activated caspase-8 in axotomized axons of the whole-mounted lamprey spinal cord. This method is based on the use of fluorochrome -labeled inhibitors of caspases (FLICA) in ex vivo tissue. We offer a very convenient vertebrate model to study the retrograde degeneration of descending pathways after spinal cord injury.

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Year:  2015        PMID： 25431075     DOI： 10.1007/978-1-4939-2152-2_23

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  RhoA activation in axotomy-induced neuronal death.

Authors:  Guixin Zhang; Jianli Hu; William Rodemer; Shuxin Li; Michael E Selzer
Journal:  Exp Neurol       Date:  2018-04-30       Impact factor: 5.330

2.  "Bad regenerators" die after spinal cord injury: insights from lampreys.

Authors:  Antón Barreiro-Iglesias
Journal:  Neural Regen Res       Date:  2015-01       Impact factor: 5.135

Review 3.  Role of Caspase-8 and Fas in Cell Death After Spinal Cord Injury.

Authors:  Daniel Sobrido-Cameán; Antón Barreiro-Iglesias
Journal:  Front Mol Neurosci       Date:  2018-04-03       Impact factor: 5.639

4.  GABA promotes survival and axonal regeneration in identifiable descending neurons after spinal cord injury in larval lampreys.

Authors:  Daniel Romaus-Sanjurjo; Rocío Ledo-García; Blanca Fernández-López; Kendra Hanslik; Jennifer R Morgan; Antón Barreiro-Iglesias; María Celina Rodicio
Journal:  Cell Death Dis       Date:  2018-06-28       Impact factor: 8.469

5.  Retrograde Activation of the Extrinsic Apoptotic Pathway in Spinal-Projecting Neurons after a Complete Spinal Cord Injury in Lampreys.

Authors:  Antón Barreiro-Iglesias; Daniel Sobrido-Cameán; Michael I Shifman
Journal:  Biomed Res Int       Date:  2017-11-19       Impact factor: 3.411

6.  Inhibition of Gamma-Secretase Promotes Axon Regeneration After a Complete Spinal Cord Injury.

Authors:  Daniel Sobrido-Cameán; Diego Robledo; Daniel Romaus-Sanjurjo; Vanessa Pérez-Cedrón; Laura Sánchez; María Celina Rodicio; Antón Barreiro-Iglesias
Journal:  Front Cell Dev Biol       Date:  2020-03-20
  6 in total

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