PURPOSE: Retinal oximetry (RO) has been established as a non-invasive method to analyse oxygen saturation in retinal vessels. The aim of our study was to determine the reproducibility of RO images in healthy and in diseased retinas. METHODS: A total of 61 right eyes (244 RO images) in 61 subjects (35♀, 26♂) were examined: 22 controls, 18 patients with glaucoma and 21 patients with inherited retinal diseases (IRDs). Four test-retest RO images were obtained in each subject. Oxygen saturation was measured with the oxygen saturation measurement tool of the Retinal Vessel Analyser (RVA; IMEDOS Systems UG, Jena, Germany). The test-retest standard deviation within the subject's (±SDw ) measurements (the mean vessel oxygen saturation in retinal venules and arterioles), its coefficient of variation (CoV) and the intraclass correlation coefficients (ICC) were analysed. RESULTS: The average test-retest SDw in venules was ±2.52% (CoV = 4.35%) and in arterioles was ±1.67% (CoV = 1.76%). Among controls, glaucoma eyes and eyes with IRDs, the test-retest SDw in venules were ±2.33% (CoV = 4.48%), ±2.85% (CoV = 4.71%) and ±2.43% (CoV = 3.90%) (SDw p = 0.366 (CoV p = 0.452); one-way anova). The test-retest SDw in arterioles were ±1.65% (CoV = 1.80%), ±1.83% (CoV = 1.92%) and ±1.54% (CoV = 1.56%), respectively [SDw p = 0.762 (CoV p = 0.686)]. The ICCs in venules were 0.76 in controls, 0.69 in patients with glaucoma and 0.82 in patients with IRD. The ICCs in arterioles were, respectively, 0.92, 0.70 and 0.93. CONCLUSION: The reproducibility of RO in healthy, as well as in diseased retinas, is excellent. In the glaucoma group, the lower standard deviation between subjects (SDb ) for arterioles contributes to the lower ICCs. Nevertheless, the measurements of oxygen saturation in arterioles seem more reliable when compared to venules.
PURPOSE: Retinal oximetry (RO) has been established as a non-invasive method to analyse oxygen saturation in retinal vessels. The aim of our study was to determine the reproducibility of RO images in healthy and in diseased retinas. METHODS: A total of 61 right eyes (244 RO images) in 61 subjects (35♀, 26♂) were examined: 22 controls, 18 patients with glaucoma and 21 patients with inherited retinal diseases (IRDs). Four test-retest RO images were obtained in each subject. Oxygen saturation was measured with the oxygen saturation measurement tool of the Retinal Vessel Analyser (RVA; IMEDOS Systems UG, Jena, Germany). The test-retest standard deviation within the subject's (±SDw ) measurements (the mean vessel oxygen saturation in retinal venules and arterioles), its coefficient of variation (CoV) and the intraclass correlation coefficients (ICC) were analysed. RESULTS: The average test-retest SDw in venules was ±2.52% (CoV = 4.35%) and in arterioles was ±1.67% (CoV = 1.76%). Among controls, glaucoma eyes and eyes with IRDs, the test-retest SDw in venules were ±2.33% (CoV = 4.48%), ±2.85% (CoV = 4.71%) and ±2.43% (CoV = 3.90%) (SDw p = 0.366 (CoV p = 0.452); one-way anova). The test-retest SDw in arterioles were ±1.65% (CoV = 1.80%), ±1.83% (CoV = 1.92%) and ±1.54% (CoV = 1.56%), respectively [SDw p = 0.762 (CoV p = 0.686)]. The ICCs in venules were 0.76 in controls, 0.69 in patients with glaucoma and 0.82 in patients with IRD. The ICCs in arterioles were, respectively, 0.92, 0.70 and 0.93. CONCLUSION: The reproducibility of RO in healthy, as well as in diseased retinas, is excellent. In the glaucoma group, the lower standard deviation between subjects (SDb ) for arterioles contributes to the lower ICCs. Nevertheless, the measurements of oxygen saturation in arterioles seem more reliable when compared to venules.
Authors: Rupesh Agrawal; Praveen Kumar Balne; Sai Bo Bo Tun; Yeo Sia Wey; Neha Khandelwal; Veluchamy A Barathi Journal: J Vis Exp Date: 2017-07-03 Impact factor: 1.355
Authors: Margarita G Todorova; Hendrik P N Scholl; Maria Della Volpe Waizel Journal: Graefes Arch Clin Exp Ophthalmol Date: 2020-09-10 Impact factor: 3.117
Authors: Rossiana I Bojinova; Daniel F Schorderet; Christophe Valmaggia; Cengiz Türksever; Andreas Schoetzau; Margarita G Todorova Journal: Eye (Lond) Date: 2018-03-06 Impact factor: 3.775
Authors: Maria Della Volpe-Waizel; Hanna Camenzind Zuche; Ursula Müller; Annekatrin Rickmann; Hendrik P N Scholl; Margarita G Todorova Journal: Graefes Arch Clin Exp Ophthalmol Date: 2019-11-12 Impact factor: 3.117