Wei Liu1, Li-Ping Shan2, Xue-Song Dong1, Zhi Liu1. 1. Department of Emergency, First Affiliated Hospital of China Medical University Shenyang, China. 2. Department of Urologic Surgery, Shengjing Hospital, China Medical University Shenyang, China.
Abstract
OBJECTIVE: To investigate the possible relationship and mechanism of Toll-like receptor 4 (TLR4) and acute lung injury induced by paraquat (PQ) poisoning. METHODS: Male wild type mice and male TLR4-knockout mice were used in this study. After paraquat treatment for 24 hours, mice were euthanized and pathology, TLR4 expression and pro-inflammatory cytokines were evaluated. RESULTS: Wild type mice showed deteriorated lung injury, pathological damages and increased TLR4 expression and pulmonary TNF-α, IL-1β and NF-κB p65 levels after PQ treatment. TLR4-deficient mice were significantly resistant to paraquat-induced lung injury. CONCLUSION: TLR4 may be required as a mediator and may play an important role in acute lung injury induced by paraquat.
OBJECTIVE: To investigate the possible relationship and mechanism of Toll-like receptor 4 (TLR4) and acute lung injury induced by paraquat (PQ) poisoning. METHODS: Male wild type mice and male TLR4-knockout mice were used in this study. After paraquat treatment for 24 hours, mice were euthanized and pathology, TLR4 expression and pro-inflammatory cytokines were evaluated. RESULTS: Wild type mice showed deteriorated lung injury, pathological damages and increased TLR4 expression and pulmonary TNF-α, IL-1β and NF-κB p65 levels after PQ treatment. TLR4-deficient mice were significantly resistant to paraquat-induced lung injury. CONCLUSION:TLR4 may be required as a mediator and may play an important role in acute lung injury induced by paraquat.
Authors: Jesús Villar; Nuria Cabrera; Milena Casula; Carlos Flores; Francisco Valladares; Mercedes Muros; Lluis Blanch; Arthur S Slutsky; Robert M Kacmarek Journal: Intensive Care Med Date: 2010-04-16 Impact factor: 17.440
Authors: A Poltorak; X He; I Smirnova; M Y Liu; C Van Huffel; X Du; D Birdwell; E Alejos; M Silva; C Galanos; M Freudenberg; P Ricciardi-Castagnoli; B Layton; B Beutler Journal: Science Date: 1998-12-11 Impact factor: 47.728