OBJECTIVES: Biofilm formation reduces the efficacy of standard microbiological techniques in prosthetic joint infection. This study aimed to investigate the sensitivity, specificity and predictive values of tissue sample enrichment as a means to increase diagnostic yield. METHODS: Patients undergoing revision arthroplasty surgery between May 2004 and January 2013 had intraoperative tissue samples cultured in standard media as well as enriched in brain heart infusion broth. Patients were separated into infected or non-infected groups according to modified criteria from the Musculoskeletal Infection Society. RESULTS: A total of 197 revision arthroplasties were included (non-infected, n = 165; proven infection, n = 32). The mean time until revision in non-infected and infected groups was 75.9 and 41.7 months, respectively. The commonest microorganisms cultured were coagulase-negative staphylococci (42.9 %) and Staphylococcus aureus (34.4 %). The sensitivity and specificity of standard tissue culture were 0.25 (CI 0.18-0.33) and 0.98 (CI 0.95-0.99), respectively. Including enrichment culture results increased the sensitivity to 0.45 (CI 0.37-0.54), but decreased specificity to 0.59 (CI 0.52-0.66). CONCLUSION: Any potential increase in the sensitivity is far outweighed by the extremely high false-positive rate. Results of tissue samples cultured by enrichment should be used with caution and may lead to a worse outcome if incorrectly interpreted.
OBJECTIVES: Biofilm formation reduces the efficacy of standard microbiological techniques in prosthetic joint infection. This study aimed to investigate the sensitivity, specificity and predictive values of tissue sample enrichment as a means to increase diagnostic yield. METHODS:Patients undergoing revision arthroplasty surgery between May 2004 and January 2013 had intraoperative tissue samples cultured in standard media as well as enriched in brain heart infusion broth. Patients were separated into infected or non-infected groups according to modified criteria from the Musculoskeletal Infection Society. RESULTS: A total of 197 revision arthroplasties were included (non-infected, n = 165; proven infection, n = 32). The mean time until revision in non-infected and infected groups was 75.9 and 41.7 months, respectively. The commonest microorganisms cultured were coagulase-negative staphylococci (42.9 %) and Staphylococcus aureus (34.4 %). The sensitivity and specificity of standard tissue culture were 0.25 (CI 0.18-0.33) and 0.98 (CI 0.95-0.99), respectively. Including enrichment culture results increased the sensitivity to 0.45 (CI 0.37-0.54), but decreased specificity to 0.59 (CI 0.52-0.66). CONCLUSION: Any potential increase in the sensitivity is far outweighed by the extremely high false-positive rate. Results of tissue samples cultured by enrichment should be used with caution and may lead to a worse outcome if incorrectly interpreted.
Authors: Alessandra Valle Daur; Francisco Klimak; Laura Lúcia Cogo; Gislene Diógenes Botão; Cristina Leise Bastos Monteiro; Libera Maria Dalla Costa Journal: Braz J Infect Dis Date: 2006-12 Impact factor: 1.949
Authors: B L Atkins; N Athanasou; J J Deeks; D W Crook; H Simpson; T E Peto; P McLardy-Smith; A R Berendt Journal: J Clin Microbiol Date: 1998-10 Impact factor: 5.948
Authors: Esa Jämsen; Markku Varonen; Heini Huhtala; Matti U K Lehto; Jukka Lumio; Yrjö T Konttinen; Teemu Moilanen Journal: J Arthroplasty Date: 2008-12-04 Impact factor: 4.757
Authors: Elie F Berbari; Camelia Marculescu; Irene Sia; Brian D Lahr; Arlen D Hanssen; James M Steckelberg; Rachel Gullerud; Douglas R Osmon Journal: Clin Infect Dis Date: 2007-09-26 Impact factor: 9.079