Ahlem Boussenna1,2, Nicolas Goncalves-Mendes3, Juliette Joubert-Zakeyh4, Bruno Pereira5, Didier Fraisse1, Marie-Paule Vasson3, Odile Texier1, Catherine Felgines6,7. 1. Unité de Nutrition Humaine, Equipe ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont Université, Université d'Auvergne, 63000, Clermont-Ferrand, France. 2. 3inature Biosphère, Parc Naturopôle, 03800, Saint-Bonnet-de-Rochefort, France. 3. Unité de Nutrition Humaine, Equipe ECREIN, Laboratoire de Biochimie Biologie Moléculaire et Nutrition, Clermont Université, Université d'Auvergne, 63000, Clermont-Ferrand, France. 4. Service d'Anatomie et de Cytologie Pathologiques, CHU de Clermont-Ferrand, 63003, Clermont-Ferrand, France. 5. Biostatistics Unit (DRCI), CHU de Clermont-Ferrand, 63003, Clermont-Ferrand, France. 6. Unité de Nutrition Humaine, Equipe ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont Université, Université d'Auvergne, 63000, Clermont-Ferrand, France. catherine.felgines@udamail.fr. 7. Laboratoire de Pharmacognosie et Phytothérapie, Faculté de Pharmacie, Clermont Université, Université d'Auvergne, 28 Place Henri-Dunant, BP 38, 63001, Clermont-Ferrand Cedex 1, France. catherine.felgines@udamail.fr.
Abstract
PURPOSE: Dextran sodium sulphate (DSS)-induced colitis is a widely used model for inflammatory bowel disease. However, various factors including nutrition may affect the development of this colitis. This study aimed to compare and characterize the impact of purified and non-purified basal diets on the development of DSS-induced colitis in the rat. METHODS: Wistar rats were fed a non-purified or a semi-synthetic purified diet for 21 days. Colitis was then induced in half of the rats by administration of DSS in drinking water (4% w/v) during the last 7 days of experimentation. At the end of the experimental period, colon sections were taken for histopathological examination, determination of various markers of inflammation (myeloperoxidase: MPO, cytokines) and oxidative stress (superoxide dismutase: SOD, catalase: CAT, glutathione peroxidase: GPx and glutathione reductase: GRed activities), and evaluation of the expression of various genes implicated in this disorder. RESULTS: DSS ingestion induced a more marked colitis in animals receiving the purified diet, as reflected by higher histological score and increased MPO activity. A significant decrease in SOD and CAT activities was also observed in rats fed the purified diet. Also, in these animals, administration of DSS induced a significant increase in interleukin (IL)-1α, IL-1β and IL-6. In addition, various genes implicated in inflammation were over-expressed after ingestion of DSS by rats fed the purified diet. CONCLUSIONS: These results show that a purified diet promotes the onset of a more severe induced colitis than a non-purified one, highlighting the influence of basal diet in colitis development.
PURPOSE:Dextran sodium sulphate (DSS)-induced colitis is a widely used model for inflammatory bowel disease. However, various factors including nutrition may affect the development of this colitis. This study aimed to compare and characterize the impact of purified and non-purified basal diets on the development of DSS-induced colitis in the rat. METHODS:Wistar rats were fed a non-purified or a semi-synthetic purified diet for 21 days. Colitis was then induced in half of the rats by administration of DSS in drinking water (4% w/v) during the last 7 days of experimentation. At the end of the experimental period, colon sections were taken for histopathological examination, determination of various markers of inflammation (myeloperoxidase: MPO, cytokines) and oxidative stress (superoxide dismutase: SOD, catalase: CAT, glutathione peroxidase: GPx and glutathione reductase: GRed activities), and evaluation of the expression of various genes implicated in this disorder. RESULTS:DSS ingestion induced a more marked colitis in animals receiving the purified diet, as reflected by higher histological score and increased MPO activity. A significant decrease in SOD and CAT activities was also observed in rats fed the purified diet. Also, in these animals, administration of DSS induced a significant increase in interleukin (IL)-1α, IL-1β and IL-6. In addition, various genes implicated in inflammation were over-expressed after ingestion of DSS by rats fed the purified diet. CONCLUSIONS: These results show that a purified diet promotes the onset of a more severe induced colitis than a non-purified one, highlighting the influence of basal diet in colitis development.
Entities:
Keywords:
Colitis; Dextran sodium sulphate; Oxidative stress; Purified diet; Rat
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