Detection of human papillomavirus type 16 DNA and papillomavirus genus-specific antigens in vulva and cervix from patients with Bowenoid papulosis.

The warty disordered lesions of the vulva in three female patients were diagnosed as Bowenoid papulosis on the basis of clinical and histopathological findings. In all three vulvar lesions, human papillomavirus type 16 (HPV 16) DNA was identified by Southern blot hybridization and papillomavirus genus-specific (PGS) antigen was detected in one case immunohistochemically. Furthermore, colposcopic examination revealed the presence of abnormal uterine cervical lesions in two cases. They were found to be intraepithelial neoplasia which harbored HPV 16 DNA and were positive for PGS antigen.