Lan Xia1, Xiaoming Zhao1, Yun Sun1, Yan Hong1, Yuping Gao1, Shuanggang Hu1. 1. Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reprodutive Medcine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
Abstract
OBJECTIVE: To establish a gas chromatography/mass spectrometry (GC/MS)-based metabolomics method to compare the metabolites in the follicular fluid (FF) from patients with in vitro fertilization (IVF) and repeated IVF failure (RIF). METHODS: A prospective study was employed in Center for Reprodutive Medcine, Renji Hospital, Shanghai, China, between January and October 2010. FF samples were collected from 13 patients with RIF and 15 patients who achieved pregnancy after the first IVF cycle. RESULTS: Partial least squares (PLS) discriminant analysis of the PCA data revealed that the samples were scattered into two different regions. FF from the two groups differed with respect to 20 metabolites. FF from RIF group showed elevated levels of several amino acids (valine, threonine, isoleucine, cysteine, serine, proline, alanine, phenylalanine, lysine, methionine and ornithine), and reduced levels of dicarboxylic acids, cholesterol and some organic acids. CONCLUSIONS: The studies corroborated successful determination of the levels of metabolite in the FF.
OBJECTIVE: To establish a gas chromatography/mass spectrometry (GC/MS)-based metabolomics method to compare the metabolites in the follicular fluid (FF) from patients with in vitro fertilization (IVF) and repeated IVF failure (RIF). METHODS: A prospective study was employed in Center for Reprodutive Medcine, Renji Hospital, Shanghai, China, between January and October 2010. FF samples were collected from 13 patients with RIF and 15 patients who achieved pregnancy after the first IVF cycle. RESULTS: Partial least squares (PLS) discriminant analysis of the PCA data revealed that the samples were scattered into two different regions. FF from the two groups differed with respect to 20 metabolites. FF from RIF group showed elevated levels of several amino acids (valine, threonine, isoleucine, cysteine, serine, proline, alanine, phenylalanine, lysine, methionine and ornithine), and reduced levels of dicarboxylic acids, cholesterol and some organic acids. CONCLUSIONS: The studies corroborated successful determination of the levels of metabolite in the FF.
Authors: C G Vergouw; L L Botros; P Roos; J W Lens; R Schats; P G A Hompes; D H Burns; C B Lambalk Journal: Hum Reprod Date: 2008-04-18 Impact factor: 6.918
Authors: R O Gottlob; J M DeRouchey; M D Tokach; R D Goodband; S S Dritz; J L Nelssen; C W Hastad; D A Knabe Journal: J Anim Sci Date: 2006-06 Impact factor: 3.159