Min Fan1, Qianfeng Zhuang1, Yiming Chen1, Tao Ding1, Hongwei Yao1, Lujun Chen2, Xiaozhou He1, Xianlin Xu1. 1. Department of Urology, The Third Affiliated Hospital of Soochow University Changzhou 213000, Jiangsu, China. 2. Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University Changzhou 213000, Jiangsu, China.
Abstract
OBJECTIVE: To investigate the mRNA and protein levels of B7-H4, a B7 family molecule, in human urothelial cell carcinoma (UCC), to analyze the relationship between B7-H4 protein expression level and pathological stage of UCC, and to examine the potential of B7-H4 as a prognostic factor in UCC. METHODS: mRNA and protein levels of B7-H4 were measured in pairs of tumor tissues and matched adjacent nontumor tissue obtained from patients with UCC by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemical staining, respectively. Association of the protein level of B7-H4 with pathological tumor stage and the overall survival of UCC patients were also analyzed. RESULTS: B7-H4 mRNA and protein level were significantly higher in UCC tumor tissues compared with adjacent nontumor tissues as assessed by qRT-PCR and immunohistochemical staining, respectively. Higher B7-H4 protein levels were observed in patients with more advanced pathological stage of UCC and were also associated with decreased overall survival of patients with UCC. CONCLUSIONS: The findings from this study indicate that B7-H4 has the potential to be an independent prognostic indicator for UCC.
OBJECTIVE: To investigate the mRNA and protein levels of B7-H4, a B7 family molecule, in humanurothelial cell carcinoma (UCC), to analyze the relationship between B7-H4 protein expression level and pathological stage of UCC, and to examine the potential of B7-H4 as a prognostic factor in UCC. METHODS: mRNA and protein levels of B7-H4 were measured in pairs of tumor tissues and matched adjacent nontumor tissue obtained from patients with UCC by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemical staining, respectively. Association of the protein level of B7-H4 with pathological tumor stage and the overall survival of UCC patients were also analyzed. RESULTS:B7-H4 mRNA and protein level were significantly higher in UCC tumor tissues compared with adjacent nontumor tissues as assessed by qRT-PCR and immunohistochemical staining, respectively. Higher B7-H4 protein levels were observed in patients with more advanced pathological stage of UCC and were also associated with decreased overall survival of patients with UCC. CONCLUSIONS: The findings from this study indicate that B7-H4 has the potential to be an independent prognostic indicator for UCC.
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