| Literature DB >> 25392234 |
Abstract
Bioinformatics skills required for genome sequencing often represent a significant hurdle for many researchers working in computational biology. This humble effort highlights the significance of genome assembly as a research area, focuses on its need to remain accurate, provides details about the characteristics of the raw data, examines some key metrics, emphasizes some tools and draws attention to a generic tutorial with example data that outlines the whole pipeline for next-generation sequencing. The article concludes by pointing out some major future research problems.Keywords: Eulerian path; comparative assembly; de novo assembly; de-Bruijn graphs; genome assembly; next-generation sequencing
Mesh:
Year: 2014 PMID: 25392234 DOI: 10.1093/bfgp/elu042
Source DB: PubMed Journal: Brief Funct Genomics ISSN: 2041-2649 Impact factor: 4.241