Literature DB >> 25391795

Fluorescence resonance energy transfer microscopy (FRET).

Katarzyna M Kedziora1, Kees Jalink.   

Abstract

FRET (Förster Resonance Energy Transfer) microscopy breaks the resolution limit of light to let us investigate the conformation and function of proteins within living cells. Intensity-based methods are the most popular and direct approach to detect FRET. Among them, detection of sensitized emission signals and ratio imaging of specially designed FRET sensors are routinely used in modern cell biology laboratories. In this chapter, we provide protocols for both these techniques. We guide the reader through the mathematical corrections necessary to calculate the sensitized emission image. We illustrate this approach with an example of studying the interaction of nexin (SNX1) proteins. In the ratio FRET protocol, we focus on monitoring changes in cellular concentration of cAMP with an EPAC-based FRET sensor.

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Year:  2015        PMID： 25391795     DOI： 10.1007/978-1-4939-2080-8_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

Review 1.  Quantifying lipid changes in various membrane compartments using lipid binding protein domains.

Authors:  Péter Várnai; Gergő Gulyás; Dániel J Tóth; Mira Sohn; Nivedita Sengupta; Tamas Balla
Journal:  Cell Calcium       Date:  2016-12-31       Impact factor: 6.817

2.  5D imaging approaches reveal the formation of distinct intracellular cAMP spatial gradients.

Authors:  Thomas C Rich; Naga Annamdevula; Kenny Trinh; Andrea L Britain; Samuel A Mayes; John R Griswold; Joshua Deal; Chase Hoffman; Savannah West; Silas J Leavesley
Journal:  Proc SPIE Int Soc Opt Eng       Date:  2017-02-17

3.  Spectral imaging of FRET-based sensors reveals sustained cAMP gradients in three spatial dimensions.

Authors:  Naga S Annamdevula; Rachel Sweat; John R Griswold; Kenny Trinh; Chase Hoffman; Savannah West; Joshua Deal; Andrea L Britain; Kees Jalink; Thomas C Rich; Silas J Leavesley
Journal:  Cytometry A       Date:  2018-09-03       Impact factor: 4.355

4.  Accepting from the best donor; analysis of long-lifetime donor fluorescent protein pairings to optimise dynamic FLIM-based FRET experiments.

Authors:  Kirsty J Martin; Ewan J McGhee; Juliana P Schwarz; Martin Drysdale; Saskia M Brachmann; Volker Stucke; Owen J Sansom; Kurt I Anderson
Journal:  PLoS One       Date:  2018-01-02       Impact factor: 3.240

5.  Use of an Isotope-Coded Mass Tag (ICMT) Method To Determine the Orientation of Cholesterol Oxidase on Model Membranes.

Authors:  John E Gadbery; Nicole S Sampson
Journal:  Biochemistry       Date:  2018-08-28       Impact factor: 3.162

6.  Transient Induced Molecular Electronic Spectroscopy (TIMES) for study of protein-ligand interactions.

Authors:  Tiantian Zhang; Ti-Hsuan Ku; Yuanyuan Han; Ramkumar Subramanian; Iftikhar Ahmad Niaz; Hua Luo; Derrick Chang; Jian-Jang Huang; Yu-Hwa Lo
Journal:  Sci Rep       Date:  2016-10-19       Impact factor: 4.379
  6 in total

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