Literature DB >> 2539157

A simple, sensitive, and specific radioreceptor assay for inositol 1,4,5-trisphosphate in biological tissues.

D S Bredt1, R J Mourey, S H Snyder.   

Abstract

Inositol (1,4,5)P3 (IP3) receptors in rat cerebellar membranes have been used to develop a radioreceptor assay for endogenous IP3. The assay is sensitive, detecting as little as 1 pmole IP3 in a 0.5 ml volume. The receptor is highly selective for the 1,4,5-isomer of IP3 so that assays can employ crude tissue extracts with no purification. Chromatographic analysis and selective enzymatic hydrolysis of IP3 show that in crude tissue extracts only authentic IP3 is detected. A hundred or more samples can be assayed in a day for endogenous IP3.

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Year:  1989        PMID： 2539157     DOI： 10.1016/0006-291x(89)92204-3

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  31 in total

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