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Literature DB >> 2538837

Mobility of the maize suppressor-mutator element in transgenic tobacco cells.

Abstract

Maize Suppressor-mutator (Spm) transposable elements have been introduced into tobacco cells and a visual assay for Spm activity has been developed using a bacterial beta-glucuronidase gene. The Spm element is mobile in tobacco and can trans-activate excision of a transposition-defective Spm (dSpm) element either from a different site on the same transforming Ti plasmid or from a second plasmid. An Spm element expressed from the stronger cauliflower mosaic virus 35S promoter trans-activates transposition of a dSpm element earlier after its introduction into tobacco cells than an element expressed from its own promoter.

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Year: 1989 PMID： 2538837 PMCID： PMC286883 DOI： 10.1073/pnas.86.7.2219

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

20 in total

1. Stable and heritable inhibition of the expression of nopaline synthase in tobacco expressing antisense RNA.

Authors: S J Rothstein; J Dimaio; M Strand; D Rice
Journal: Proc Natl Acad Sci U S A Date: 1987-12 Impact factor: 11.205

2. Is the Suppressor-mutator element controlled by a basic developmental regulatory mechanism?

Authors: N V Fedoroff; J A Banks
Journal: Genetics Date: 1988-10 Impact factor: 4.562

3. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors: R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal: Science Date: 1985-12-20 Impact factor: 47.728

9. Functional analysis of the 3'-terminal sequence of the maize controlling element (Ac) by internal replacement and deletion mutagenesis.

Authors: J H Zhou; A Myers; A G Atherly
Journal: Genetica Date: 1991 Impact factor: 1.082

Review 10. The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies.

Authors: M A Haring; C M Rommens; H J Nijkamp; J Hille
Journal: Plant Mol Biol Date: 1991-03 Impact factor: 4.076

Mobility of the maize suppressor-mutator element in transgenic tobacco cells.

1. Stable and heritable inhibition of the expression of nopaline synthase in tobacco expressing antisense RNA.

2. Is the Suppressor-mutator element controlled by a basic developmental regulatory mechanism?

3. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

4. Transposition of the maize controlling element "Activator" in tobacco.

5. Transformation of Arabidopsis thaliana with Agrobacterium tumefaciens.

6. Engineering herbicide tolerance in transgenic plants.

7. Molecular analysis of the En/Spm transposable element system of Zea mays.

8. Molecular interactions between the components of the En-I transposable element system of Zea mays.

9. Phenotypic assay for excision of the maize controlling element Ac in tobacco.

10. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

1. A four-element based transposon system for allele specific tagging in plants--theoretical considerations.

2. Survival of Tdc transposable elements of the En/Spm superfamily in the carrot genome.

3. Inducible DNA demethylation mediated by the maize Suppressor-mutator transposon-encoded TnpA protein.

4. Resources for targeted insertional and deletional mutagenesis in Arabidopsis.

5. Directed excision of a transgene from the plant genome.

6. Transcription and somatic transposition of the maize En/Spm transposon system in rice.

7. A transcript identified by MuA of maize is associated with Mutator activity.

8. The tnpA and tnpD gene products of the Spm element are required for transposition in tobacco.

9. Functional analysis of the 3'-terminal sequence of the maize controlling element (Ac) by internal replacement and deletion mutagenesis.

Review 10. The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies.