Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The tnpA and tnpD gene products of the Spm element are required for transposition in tobacco.

Literature DB >> 1668614

The tnpA and tnpD gene products of the Spm element are required for transposition in tobacco.

Abstract

The maize Suppressor-mutator (Spm) element encodes four alternatively spliced transcripts designated tnpA, tnpB, tnpC, and tnpD. tnpA and tnpB are monocistronic, whereas tnpC and tnpD are dicistronic, and the protein-coding sequences of each transcript overlap extensively with those of one or more of the other transcripts. We have analyzed the role of the Spm-encoded gene products in element transposition by using cDNAs with a single open reading frame to (1) complement Spm elements with frameshift mutations and (2) complement each other in a tobacco transposition assay. We report that whereas the tnpA and tnpD gene products are essential for transposition, the tnpB and tnpC gene products are not. We have analyzed the structure of empty donor sites, new insertion sites, and potential transposition intermediates. We discuss the implications of our findings for the mechanism of Spm transposition.

Entities: Species

Mesh：

Substances：

Year: 1991 PMID： 1668614 PMCID： PMC159980 DOI： 10.1105/tpc.3.1.73

Source DB: PubMed Journal: Plant Cell ISSN： 1040-4651 Impact factor: 11.277

15 in total

1. Identification and immunochemical analysis of biologically active Drosophila P element transposase.

Authors: D C Rio; F A Laski; G M Rubin
Journal: Cell Date: 1986-01-17 Impact factor: 41.582

2. Essential large transcripts of the maize Spm transposable element are generated by alternative splicing.

Authors: P Masson; G Rutherford; J A Banks; N Fedoroff
Journal: Cell Date: 1989-08-25 Impact factor: 41.582

3. Genetic and molecular analysis of the Spm-dependent a-m2 alleles of the maize a locus.

Authors: P Masson; R Surosky; J A Kingsbury; N V Fedoroff
Journal: Genetics Date: 1987-09 Impact factor: 4.562

4. Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus.

Authors: E S Coen; R Carpenter; C Martin
Journal: Cell Date: 1986-10-24 Impact factor: 41.582

5. Mobility of the maize suppressor-mutator element in transgenic tobacco cells.

Authors: P Masson; N V Fedoroff
Journal: Proc Natl Acad Sci U S A Date: 1989-04 Impact factor: 11.205

6. Expression of a truncated viral trans-activator selectively impedes lytic infection by its cognate virus.

Authors: A D Friedman; S J Triezenberg; S L McKnight
Journal: Nature Date: 1988-09-29 Impact factor: 49.962

7. Molecular characterization of suppressor-mutator (Spm)-induced mutations at the bronze-1 locus in maize: the bz-m13 alleles.

Authors: J W Schiefelbein; V Raboy; H Y Kim; O E Nelson
Journal: Basic Life Sci Date: 1988

8. Sequence of three bronze alleles of maize and correlation with the genetic fine structure.

Authors: E J Ralston; J J English; H K Dooner
Journal: Genetics Date: 1988-05 Impact factor: 4.562

9. En/Spm encoded tnpA protein requires a specific target sequence for suppression.

Authors: S R Grant; A Gierl; H Saedler
Journal: EMBO J Date: 1990-07 Impact factor: 11.598

10. TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein.

Authors: A Gierl; S Lütticke; H Saedler
Journal: EMBO J Date: 1988-12-20 Impact factor: 11.598

31 in total

1. Somatic and germinal excision activities of the Arabidopsis transposon Tag1 are controlled by distinct regulatory sequences within Tag1.

Authors: D Liu; R Wang; M Galli; N M Crawford
Journal: Plant Cell Date: 2001-08 Impact factor: 11.277

The tnpA and tnpD gene products of the Spm element are required for transposition in tobacco.

1. Identification and immunochemical analysis of biologically active Drosophila P element transposase.

2. Essential large transcripts of the maize Spm transposable element are generated by alternative splicing.

3. Genetic and molecular analysis of the Spm-dependent a-m2 alleles of the maize a locus.

4. Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus.

5. Mobility of the maize suppressor-mutator element in transgenic tobacco cells.

6. Expression of a truncated viral trans-activator selectively impedes lytic infection by its cognate virus.

7. Molecular characterization of suppressor-mutator (Spm)-induced mutations at the bronze-1 locus in maize: the bz-m13 alleles.

8. Sequence of three bronze alleles of maize and correlation with the genetic fine structure.

9. En/Spm encoded tnpA protein requires a specific target sequence for suppression.

10. TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein.

1. Somatic and germinal excision activities of the Arabidopsis transposon Tag1 are controlled by distinct regulatory sequences within Tag1.

2. Survival of Tdc transposable elements of the En/Spm superfamily in the carrot genome.

3. Inducible DNA demethylation mediated by the maize Suppressor-mutator transposon-encoded TnpA protein.

Review 4. Plant-transposable elements and gene tagging.

5. Transcription and somatic transposition of the maize En/Spm transposon system in rice.

6. Suppression of an atypically spliced rice CACTA transposon transcript in transgenic plants.

7. Mutator activity in maize correlates with the presence and expression of the Mu transposable element Mu9.

8. Novel DNA structures resulting from dTam3 excision in tobacco.

9. The inveterate wanderer: study of Enhancer wandering on chromosome 3 in maize.

10. The binding motifs for Ac transposase are absolutely required for excision of Ds1 in maize.